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Zeinab Rafiee. Picture.

Zeinab Rafiee

Zeinab Rafiee, Postdoctoral researcher

Zeinab Rafiee. Picture.

Effect of kidney stem cells on inflammatory factors in rats with diabetic nephropathy

Author

  • Zeinab Rafiee
  • Mahmoud Orazizadeh
  • Esrafil Mansouri

Summary, in English

Mesenchymal stem cells (MSCs) were proposed as a critical therapeutic candidate in diabetic nephropathy (DN). Renal stem cells as a source for repairing are controversial. The purpose of the present study was to evaluate the effect of kidney rat stem cells on DN. After evaluation of surface stem cell markers by flow cytometry analysis, MSCs that were labeled with CM-Dil were injected to experimental groups via tail vein (2×106cells/rat). To establish the diabetic nephropathy rat model STZ(60mg/kg) was used. The IL1-β, TGF-β, miRNA 192, miRNA29a gene expression were assessed three weeks after induction of early diabetic nephropathy by using quantitative Real-time PCR. The renal stem cells 24 h after labeling with CM-Dil fluorescent dye were detected in the renal tissues. miRNA192, TGF-β and IL1-β Genes expression upregulated in diabetic group. The expression of miRNA29a significantly down-regulated 21 days after injection of STZ. H and E staining showed in the presence of adult kidney stem cells histopathological changes were improved. The protective effect of MSCs was closely related to the inactivation of inflammatory factors. Therefore, the MSCs may be a promising novel treatment for early DN.

Publishing year

2021

Language

English

Pages

1091-1091

Document type

Conference paper: abstract

Keywords

  • stem cell therapy
  • Inflammatory cytokines
  • Diabetic nephropathy

Conference name

6th world congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS2021)

Conference date

2021-11-15 - 2021-11-19

Conference place

Maastricht, Netherlands

Status

Published