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Zeinab Rafiee. Picture.

Zeinab Rafiee

Zeinab Rafiee, Postdoctoral researcher

Zeinab Rafiee. Picture.

Taurine effects on bisphenol a-induced oxidative stress in the mouse testicular mitochondria and sperm motility

Author

  • Fatemeh Rezaee-Tazangi
  • Leila Zeidooni
  • Zeinab Rafiee
  • Fereshtesadat Fakhredini
  • Heybatollah Kalantari
  • Hadis Alidadi
  • Layasadat Khorsandi

Summary, in English

Objectives: This study was performed to investigate the protective effects of taurine (2-aminoethanesulfonic acid, TAU) on oxidative stress in the isolated mouse testicular mitochondria, mitochondrial membrane potential (MMP), viability and motility of the exposed sperms to the BPA. Methods: We treated epididymal spermatozoa obtained from mice and isolated mouse testicular mitochondria with BPA (0.8 mmol/mL) and various doses of TAU (5, 10, 30 and 50 µmol/L). We used the MTT assay and Rhodamine 123 uptake to assess sperm viability and MMP. We assessed the oxidative stress through measuring ROS (reactive oxygen species), MDA (malondialdehyde), GSH (glutathione), and SOD (super-oxide dismutase) levels in the testicular mitochondrial tissue. Results: BPA significantly elevated ROS, MDA and MMP levels, and markedly reduced SOD and GSH levels in the isolated mitochondria. BPA also considerably impaired spermatozoa viability and motility. Pretreatment with 30 and 50 µmol/L of TAU could considerably suppressed mitochondrial oxidative stress, enhanced MMP, and improved sperm motility and viability. Conclusion: TAU may attenuate the BPA-induced mitochondrial toxicity and impaired sperm motility via decreasing oxidative stress.

Publishing year

2020

Language

English

Pages

428-435

Publication/Series

Jornal Brasileiro de Reproducao Assistida

Volume

24

Issue

4

Document type

Journal article

Publisher

Sociedade Brasileira de Reproducao Assistida

Topic

  • Developmental Biology

Keywords

  • Bisphenol A
  • Mitochondria
  • Oxidative stress
  • Sperm motility
  • Taurine

Status

Published

ISBN/ISSN/Other

  • ISSN: 1517-5693