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Epigenetic alterations in blood mirror age-associated DNA methylation and gene expression changes in human liver

Author:
  • Madhusudhan Bysani
  • Alexander Perfilyev
  • Vanessa de Mello
  • Tina Rönn
  • Emma Nilsson
  • Jussi Pihlajamäki
  • Charlotte Ling
Publishing year: 2017-02-01
Language: English
Pages: 105-122
Publication/Series: Epigenomics
Volume: 9
Issue: 2
Document type: Journal article
Publisher: Future Medicine Ltd.

Abstract english

Aim: To study the impact of aging on DNA methylation and mRNA expression in human liver. Experimental procedures: We analysed genome-wide DNA methylation and gene expression in human liver samples using Illumina 450K and HumanHT12 expression BeadChip arrays. Results: DNA methylation analysis of ∼455,000 CpG sites in human liver revealed that age was significantly associated with altered DNA methylation of 20,396 CpG sites. Comparison of liver methylation data with published methylation data in other tissues showed that vast majority of the age-associated significant CpG sites overlapped between liver and blood, whereas a smaller overlap was found between liver and pancreatic islets or adipose tissue, respectively. We identified 151 genes whose liver expression also correlated with age. Conclusions: We identified age-associated DNA methylation and expression changes in human liver that are partly reflected by epigenetic alterations in blood.

Keywords

  • Medical Genetics
  • aging
  • blood
  • diabetes
  • DNA methylation
  • ELOVL2
  • epigenetics
  • FHL2
  • gene expression
  • KLF14
  • liver

Other

Published
  • Epigenetics and Diabetes
  • ISSN: 1750-1911
Tina Rönn
E-mail: tina [dot] ronn [at] med [dot] lu [dot] se

Assistant researcher

Epigenetics and Diabetes

+46 40 39 12 18

CRC 91-12-021

33

Lund University Diabetes Centre, CRC, SUS Malmö, Entrance 72, House 91:12. SE-205 02 Malmö. Telephone: +46 40 39 10 00