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Tereza Planck

Physician

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Thyrostimulin (a TSH-like Hormone) Expression in Orbital and Thyroid Tissue.

Author

  • Mikael Lantz
  • Tereza Planck
  • Annika Capretz
  • Emma Nilsson
  • Christofer Frenander
  • Anne-Greth Bondeson
  • Martin Ridderstråle
  • Magnus Åberg
  • Peter Åsman
  • Leif Groop
  • Bengt Hallengren

Summary, in English

Objective: To investigate gene expression of thyrostimulin in orbital and thyroid tissue from patients with and without Graves' disease. Design: Real-time reverse transcriptase polymerase chain reaction (RT-PCR) was used for detection of thyrostimulin gene expression in intraorbital adipose tissue from patients with severe ophthalmopathy and thyroid healthy controls in addition to thyrostimulin expression in normal thyroid tissue, multinodular goiter tissue, and Graves' thyroid tissue. Main Outcome: In intraorbital tissue, thyrostimulin expression was identified in both patients and controls with fluorescence intensities varying between 0.23 and 0.88 in patients and 0.29 and 8.9 in controls before treatment with DNase. The signal of thyrostimulin was weak or absent in intraorbital adipose tissue from patients with ophthalmopathy and thyroid healthy controls after treatment of samples with DNase. This was in contrast to the expression of the thyroid-stimulating hormone (TSH) receptor and the housekeeping gene cyclophilin A that were detected both before and after DNase treatment. Similar results were found when analyzing human and rat thyroid tissue. Conclusions: Neither did we demonstrate gene expression of thyrostimulin in intraorbital adipose tissue or in thyroid tissue, nor could we confirm earlier findings in rat thyroid tissue. Whether thyrostimulin is a regulator of thyroid function has to be further investigated in future studies.

Department/s

  • Genomics, Diabetes and Endocrinology
  • Department of Clinical Sciences, Malmö
  • Diabetes - Clinical Obesity
  • Ophthalmology (Malmö)

Publishing year

2007

Language

English

Pages

113-118

Publication/Series

Thyroid

Volume

17

Issue

2

Document type

Journal article

Publisher

Mary Ann Liebert, Inc.

Topic

  • Endocrinology and Diabetes

Status

Published

Research group

  • Genomics, Diabetes and Endocrinology
  • Diabetes - Clinical Obesity
  • Ophthalmology (Malmö)

ISBN/ISSN/Other

  • ISSN: 1557-9077