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Sebastian Kalamajski

Assistant researcher

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Fibromodulin binds collagen type I via Glu-353 and Lys-355 in leucine-rich repeat 11

Author

  • Sebastian Kalamajski
  • Åke Oldberg

Summary, in English

Fibromodulin belongs to the small leucine-rich repeat proteoglycan family, interacts with collagen type I, and controls collagen fibrillogenesis and assembly. Here, we show that a major fibromodulin-binding site for collagen type I is located in leucine-rich repeat 11 in the C terminus of the leucine-rich repeat domain. We identified Glu-353 and Lys-355 in repeat 11 as essential for binding, and the synthetic peptide RLDGNEIKR, including Glu-353 and Lys-355, inhibits the binding of fibromodulin to collagen in vitro. Fibromodulin and lumican compete for the same binding region on collagen, and fibromodulin can inhibit the binding of lumican to collagen type I. However, the peptide RLDGNEIKR does not inhibit the binding of lumican to collagen, suggesting separate but closely situated fibromodulin- and lumican-binding sites in collagen. The collagen-binding Glu-353 and Lys-355 residues in fibromodulin are exposed on the exterior of the beta-sheet-loop structure of the leucine-rich repeat, which resembles the location of interacting residues in other leucine-rich repeat proteins, e. g. decorin.

Department/s

  • Åke Oldberg´s group

Publishing year

2007

Language

English

Pages

26740-26745

Publication/Series

Journal of Biological Chemistry

Volume

282

Issue

37

Document type

Journal article

Publisher

American Society for Biochemistry and Molecular Biology

Topic

  • Cell and Molecular Biology

Keywords

  • Fibromodulin
  • collagen
  • Glu353
  • repeat 11
  • Lys355
  • type I
  • leucine-rich

Status

Published

Research group

  • Åke Oldberg´s group

ISBN/ISSN/Other

  • ISSN: 1083-351X