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Albert Salehi

S Albert Salehi

Research team manager

Albert Salehi

Uridine diphosphate (UDP) stimulates insulin secretion by activation of P2Y(6) receptors.

Author

  • Fariborz Parandeh
  • Sandra Meidute Abaraviciene
  • Stefan Amisten
  • David Erlinge
  • S Albert Salehi

Summary, in English

We examined the transcriptional expression and functional effects of receptors for the extracellular pyrimidines uridine triphosphate (UTP) and uridine diphosphate (UDP), on insulin and glucagon secretion in isolated mouse pancreatic islets and purified beta-cells. Using real-time PCR, the UDP receptor P2Y(6) was found to be highly expressed in both whole islets and beta-cells purified by repeated counter-flow elutriation, whereas no mRNA expression for UTP receptors P2Y(4) and P2Y(2) could be detected. Functional in vitro experiments revealed that the P2Y(6) agonist UDPbetaS dose-dependently enhanced insulin and glucagon release during short-term incubation (1h), while P2Y(6) activation during a longer period (24h), selectively increased insulin release, especially at high glucose levels. The corresponding EC(50) value for UDPbetaS ranged from 3.2x10(-8)M to 1.6x10(-8)M for both glucose concentrations. The P2Y(6) antagonist MRS2578 inhibited the effects of UDPbetaS, supporting a P2Y(6) specific effect. In addition to negative RT-PCR results, the lack of response to UTPgammaS a selective P2Y(2/4) agonist further rule out the involvement of P2Y(2/4) receptors in the islet hormone release. Our results suggest a modulatory role for UDP via a functional active P2Y(6) receptor in the regulation of islet hormone release.

Department/s

  • Cardiology
  • Islet cell physiology

Publishing year

2008

Language

English

Pages

499-503

Publication/Series

Biochemical and Biophysical Research Communications

Volume

6;370

Issue

3

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Status

Published

Research group

  • Islet cell physiology

ISBN/ISSN/Other

  • ISSN: 1090-2104