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Albert Salehi

S Albert Salehi

Research team manager

Albert Salehi

ADP receptor P2Y(13) induce apoptosis in pancreatic beta-cells.

Author

  • Chanyuan Tan
  • S Albert Salehi
  • Siv Svensson
  • Björn Olde
  • David Erlinge

Summary, in English

Pancreatic beta-cell loss represents a key factor in the pathogenesis of diabetes. Since the influence of purinergic signaling in beta-cell apoptosis has not been much investigated, we examined the role of the ADP receptor P2Y(13) using the pancreatic insulinoma-cell line MIN6c4 as a model system. Real time-PCR revealed high expression of the ADP receptors P2Y(1) and P2Y(13). Adding the ADP analogue, 2MeSADP, to MIN6c4 cells induced calcium influx/mobilization and inhibition of cAMP production by activation of P2Y(1) and P2Y(13), respectively. 2MeSADP reduced cell proliferation and increased Caspase-3 activity; both these effects could be fully reversed by the P2Y(13) receptor antagonist MRS2211. We further discovered that blocking the P2Y(13) receptor results in enhanced ERK1/2, Akt/PKB and CREB phosphorylation mechanisms involved in beta-cell survival. These results indicate that P2Y(13) is a proapoptotic receptor in beta-cells as the P2Y(13) receptor antagonist MRS2211 is able to protect the cells from ADP induced apoptosis.

Department/s

  • Cardiology
  • Islet cell physiology
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2010

Language

English

Pages

445-453

Publication/Series

Cellular and Molecular Life Sciences

Volume

67

Document type

Journal article

Publisher

Birkhäuser Verlag

Topic

  • Cell Biology

Status

Published

Research group

  • Islet cell physiology

ISBN/ISSN/Other

  • ISSN: 1420-9071