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Albert Salehi

S Albert Salehi

Research team manager

Albert Salehi

GPRC5B a putative glutamate-receptor candidate is negative modulator of insulin secretion

Author

  • Arvind Soni
  • Stefan Amisten
  • Patrik Rorsman
  • S Albert Salehi

Summary, in English

GPRC5B is an orphan receptor belonging to the group C family of G protein-coupled receptors (GPCRs). GPRC5B is abundantly expressed in both human and mouse pancreatic islets, and both GPRC5B mRNA and protein are up-regulated 2.5-fold in islets from organ donors with type 2 diabetes. Expression of Gprc5b is 50% lower in islets isolated from newborn (<3 weeks) than in adult (>36 weeks) mice. Lentiviral shRNA-mediated down-regulation of Gprc5b in intact islets from 12 to 16 week-old mice strongly (2.5-fold) increased basal (I mmol/l) and moderately (40%) potentiated glucose (20 mmol/l) stimulated insulin secretion and also enhanced the potentiating effect of glutamate on insulin secretion. Downregulation of Gprc5b protected murine insulin-secreting clonal MIN6 cells against cytokine-induced apoptosis. We propose that increased expression of GPRC5B contributes to the reduced insulin secretion and beta-cell viability observed in type-2 diabetes. Thus, pharmacological targeting of GPRC5B might provide a novel means therapy for the treatment and prevention of type-2 diabetes. (C) 2013 Elsevier Inc. All rights reserved.

Department/s

  • Islet cell physiology
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2013

Language

English

Pages

643-648

Publication/Series

Biochemical and Biophysical Research Communications

Volume

441

Issue

3

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • Diabetes
  • Endocrinology
  • Pancreatic islet
  • Insulin secretion
  • Cell
  • viability

Status

Published

Research group

  • Islet cell physiology

ISBN/ISSN/Other

  • ISSN: 1090-2104