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Rui Simoes. Picture.

Rui Simoes

Rui Simöes, Guest researcher

Rui Simoes. Picture.

Afrostyrax lepidophyllus Mildbr. and Monodora myristica (Gaertn.) Dunal Extracts Decrease Doxorubicin Cytotoxicity on H9c2 Cardiomyoblasts

Author

  • Bruno M Moukette
  • José P Castelão-Baptista
  • Luciana Ferreira
  • Ana M Silva
  • Rui F Simões
  • Célia Cabral
  • Constant A Pieme
  • Jeanne Y Ngogang
  • Vilma A Sardão
  • Paulo J Oliveira

Summary, in English

Materials and Methods: Bark extracts of these plants (1 and 25 µg/mL) were added 3 hours before coincubating H9c2 cardiomyoblasts with Dox (0.5 and 1 µM) for 24 hours more. We measured cell mass and metabolic viability, mitochondrial transmembrane potential, superoxide anion content, and activity-like of caspase-3 and caspase-9 following treatment with the extracts and/or Dox. Also, selenium and vitamin C contents were measured in the plant extracts.

Results: The results confirmed that Dox treatment decreased cell mass, mitochondrial membrane potential and metabolic viability, increased mitochondrial superoxide anion, and stimulated caspase-3 and caspase-9-like activities. Pretreatment of the cells with the plant extracts significantly inhibited Dox cytotoxicity, with more significant results at the higher concentration. Measurements of selenium and vitamin C in the extracts revealed higher concentration of both when compared with other Cameroonian spices.

Conclusion: Both extracts of A. lepidophyllus and M. myristica were effective against Dox-induced cytotoxicity, most likely due to their content in antioxidants.

Publishing year

2021

Language

English

Publication/Series

Evidence-based Complementary and Alternative Medicine

Volume

2021

Document type

Journal article

Publisher

Hindawi Limited

Topic

  • Cell and Molecular Biology
  • Pharmacology and Toxicology

Status

Published

ISBN/ISSN/Other

  • ISSN: 1741-427X