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Continuous full filling capillary electrochromatography-electrospraying chromatographic nanoparticles

Author:
  • David Malmstrom
  • Jakob Axen
  • Jonas Bergquist
  • Peter Viberg
  • Peter Spégel
Publishing year: 2011
Language: English
Pages: 261-267
Publication/Series: Electrophoresis
Volume: 32
Issue: 2
Document type: Journal article
Publisher: John Wiley & Sons

Abstract english

The influence of instrumental parameters affecting the ionization in continuous full filling capillary electrochromatography/electrospray ionization mass spectrometry (CFF-CEC/ESI-MS) was investigated. The investigated parameters were the BGE and sheath liquid ion strength and organic modifier content, the nebulizer gas pressure, and the concentration of nanoparticles in the BGE. It was found that the nebulizer pressure had the largest influence on the separation efficiency and apparent retention. It was shown that even the lowest pressure investigated was sufficient to guide the nanoparticle flow away from the mass spectrometer inlet. A nebulizer pressure of 5 psi was found to be optimal; increasing the pressure significantly decreased the separation efficiency due to the generation of a hydrodynamic flow. Generally, the ion strength of both the BGE and the sheath liquid were found to have very moderate effects on the separation of a homologous series of dialkyl phthalates, whereas the ionization efficiency was found to be unaffected by the nanoparticles and the separation efficiency was found to increase with increasing concentrations up to 3.8 mg/mL, whereafter it was observed to drop. The optimized method was linear over a wide concentration range and presented LOD and LOQ more than threefold lower than those previously reported using CFF-CEC/ESI-MS.

Keywords

  • Endocrinology and Diabetes
  • Continuous full filling capillary electrochromatography
  • Nanoparticles
  • Nebulizing gas pressure
  • Pseudostationary phase
  • Sheath liquid

Other

Published
  • Molecular Metabolism
  • ISSN: 0173-0835
Peter Spegel
E-mail: peter [dot] spegel [at] chem [dot] lu [dot] se

Researcher

Centre for Analysis and Synthesis

1

Lund University Diabetes Centre, CRC, SUS Malmö, Entrance 72, House 91:12. SE-205 02 Malmö. Telephone: +46 40 39 10 00