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Continuous full filling capillary electrochromatography: Chromatographic performance and reproducibility

Author:
  • Peter Viberg
  • Peter Spégel
  • Jonas Carlstedt
  • Magnus Jornten-Karlsson
  • Patrik Petersson
Publishing year: 2007
Language: English
Pages: 386-389
Publication/Series: Journal of Chromatography A
Volume: 1154
Issue: 1-2
Document type: Journal article
Publisher: Elsevier

Abstract english

Continuous full filling capillary electrochromatography with nanoparticles as pseudostationary phase interfaced with electrospray ionisation mass spectrometric detection was used for reversed phase separations with very high separation efficiency. Several batches of nanoparticles were synthesised and their electrochromatographic performance were evaluated. Different parameters, such as repeatability, reproducibility, limit of detection, and peak asymmetry, were investigated yielding excellent results. The stability of the system over wide pH ranges and over time was found to be excellent. Very high separation efficiencies with over 1.1 million theoretical plates per metre were obtained. The limit of detection for the investigated dialkyl phthalates was approximately 1.0 mu mol L-1, corresponding to 3-5 fmol injected. After preparation, nanoparticle suspensions could be used without further treatment for at least an entire working day with maintained chromatographic qualities. (c) 2007 Elsevier B.V. All rights reserved.

Keywords

  • Physical Chemistry
  • Analytical Chemistry
  • pseudostationary phase
  • nanoparticles
  • electrospray ionisation
  • orthogonal
  • capillary electrochromatography
  • mass spectrometry
  • reversed phase
  • continuous full filling

Other

Published
  • ISSN: 0021-9673
Peter Spegel
E-mail: peter [dot] spegel [at] chem [dot] lu [dot] se

Researcher

Centre for Analysis and Synthesis

1

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