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Ultrasonic-trap-enhanced selectivity in capillary electrophoresis.

  • M Wiklund
  • Peter Spégel
  • Staffan Nilsson
  • H M Hertz
Publishing year: 2003
Language: English
Pages: 329-333
Publication/Series: Ultrasonics
Volume: 41
Issue: 4
Document type: Journal article
Publisher: Elsevier

Abstract english

We combine ultrasonic trapping and capillary electrophoresis (CE) with the goal to detect ultra-low concentrations of proteins via size-selective separation and enrichment of antibody-coated latex spheres. An 8.5 MHz standing ultrasonic wave is longitudinally coupled into the sub-100-small mu, Greekm diam capillary of the CE system. Competition between acoustic and viscous forces result in in-flow separation of small mu, Greekm-diam spheres according to their size. Experiments separating 2.8- and 2.1-small mu, Greekm-diam fluorescent latex particles, which model a protein-specific immunocomplex/free particle mixture, indicate a potential improvement of the concentration limit of detection of 104 compared to current CE systems. Theoretical calculations show room for further improvement.


  • Analytical Chemistry
  • Ultrasonic trapping
  • Capillary electrophoresis
  • Particle separation
  • Concentration limit of detection


  • ISSN: 0041-624X
Peter Spegel
E-mail: peter [dot] spegel [at] chem [dot] lu [dot] se


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