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Peter Spégel

Principal investigator

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Continuous full filling capillary electrochromatography-electrospraying chromatographic nanoparticles

Author

  • David Malmstrom
  • Jakob Axen
  • Jonas Bergquist
  • Peter Viberg
  • Peter Spégel

Summary, in English

The influence of instrumental parameters affecting the ionization in continuous full filling capillary electrochromatography/electrospray ionization mass spectrometry (CFF-CEC/ESI-MS) was investigated. The investigated parameters were the BGE and sheath liquid ion strength and organic modifier content, the nebulizer gas pressure, and the concentration of nanoparticles in the BGE. It was found that the nebulizer pressure had the largest influence on the separation efficiency and apparent retention. It was shown that even the lowest pressure investigated was sufficient to guide the nanoparticle flow away from the mass spectrometer inlet. A nebulizer pressure of 5 psi was found to be optimal; increasing the pressure significantly decreased the separation efficiency due to the generation of a hydrodynamic flow. Generally, the ion strength of both the BGE and the sheath liquid were found to have very moderate effects on the separation of a homologous series of dialkyl phthalates, whereas the ionization efficiency was found to be unaffected by the nanoparticles and the separation efficiency was found to increase with increasing concentrations up to 3.8 mg/mL, whereafter it was observed to drop. The optimized method was linear over a wide concentration range and presented LOD and LOQ more than threefold lower than those previously reported using CFF-CEC/ESI-MS.

Department/s

  • Diabetes - Molecular Metabolism
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2011

Language

English

Pages

261-267

Publication/Series

Electrophoresis

Volume

32

Issue

2

Document type

Journal article

Publisher

John Wiley & Sons Inc.

Topic

  • Endocrinology and Diabetes

Keywords

  • Continuous full filling capillary electrochromatography
  • Nanoparticles
  • Nebulizing gas pressure
  • Pseudostationary phase
  • Sheath liquid

Status

Published

Research group

  • Diabetes - Molecular Metabolism

ISBN/ISSN/Other

  • ISSN: 0173-0835