Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Olof Gidlöf

Research project participant

Default user image.

Association of serum MiR-142-3p and MiR-101-3p levels with acute cellular rejection after heart transplantation

Author

  • Ihdina Sukma Dewi
  • Zsuzsanna Hollander
  • Karen K. Lam
  • Janet Wilson McManus
  • Scott J. Tebbutt
  • Raymond T. Ng
  • Paul A. Keown
  • Robert W. McMaster
  • Bruce M. McManus
  • Olof Gidlöf
  • Jenny Öhman

Summary, in English

Background: Identifying non-invasive and reliable blood-derived biomarkers for early detection of acute cellular rejection in heart transplant recipients is of great importance in clinical practice. MicroRNAs are small molecules found to be stable in serum and their expression patterns reflect both physiological and underlying pathological conditions in human. Methods: We compared a group of heart transplant recipients with histologically-verified acute cellular rejection (ACR, n = 26) with a control group of heart transplant recipients without allograft rejection (NR, n = 37) by assessing the levels of a select set of microRNAs in serum specimens. Results: The levels of seven microRNAs, miR-142-3p, miR-101-3p, miR-424-5p, miR-27a-3p, miR-144-3p, miR-339-3p and miR-326 were significantly higher in ACR group compared to the control group and could discriminate between patients with and without allograft rejection. MiR-142-3p and miR-101-3p had the best diagnostic test performance among the microRNAs tested. Serum levels of miR-142-3p and miR-101-3p were independent of calcineurin inhibitor levels, as measured by tacrolimus and cyclosporin; kidney function, as measured by creatinine level, and general inflammation state, as measured by CRP level. Conclusion: This study demonstrated two microRNAs, miR-142-3p and miR-101-3p, that could be relevant as non-invasive diagnostic tools for identifying heart transplant patients with acute cellular rejection.

Department/s

  • Cardiology

Publishing year

2017

Language

English

Publication/Series

PLoS ONE

Volume

12

Issue

1

Document type

Journal article

Publisher

Public Library of Science

Topic

  • Clinical Laboratory Medicine

Status

Published

ISBN/ISSN/Other

  • ISSN: 1932-6203