Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Olle Melander

Principal investigator

Default user image.

Effect of acute hypohydration on glycemic regulation in healthy adults : A randomized crossover trial

Author

  • Harriet A. Carroll
  • Iain Templeman
  • Yung Chih Chen
  • Robert M. Edinburgh
  • Elaine K. Burch
  • Jake T. Jewitt
  • Georgie Povey
  • Timothy D. Robinson
  • William L. Dooley
  • Robert Jones
  • Kostas Tsintzas
  • Widet Gallo
  • Olle Melander
  • Dylan Thompson
  • Lewis J. James
  • Laura Johnson
  • James A. Betts

Summary, in English


he aim of this study was to investigate the acute effect of hydration status on glycemic regulation in healthy adults and explore underlying mechanisms. In this randomized crossover trial, 16 healthy adults (8 men, 8 women) underwent an oral glucose tolerance test (OGTT) when hypohydrated and rehydrated after 4 days of pretrial standardization. One day before OGTT, participants were dehydrated for 1 h in a heat tent with subsequent fluid restriction (HYPO) or replacement (RE). The following day, an OGTT was performed with metabolic rate measurements and pre- and post-OGTT muscle biopsies. Peripheral quantitative computer tomography thigh scans were taken before and after intervention to infer changes in cell volume. HYPO (but not RE) induced 1.9% (SD 1.2) body mass loss, 2.9% (SD 2.7) cell volume reduction, and increased urinary hydration markers, serum osmolality, and plasma copeptin concentration (all P 0.007). Fasted serum glucose [HYPO 5.10 mmol/l (SD 0.42), RE 5.02 mmol/l (SD 0.40); P 0.327] and insulin [HYPO 27.1 pmol/l (SD 9.7), RE 27.6 pmol/l (SD 9.2); P 0.809] concentrations were similar between HYPO and RE. Hydration status did not alter the serum glucose (P 0.627) or insulin (P 0.200) responses during the OGTT. Muscle water content was lower before OGTT after HYPO compared with RE [761 g/kg wet wt (SD 13) vs. 772 g/kg wet wt (SD 18) RE] but similar after OGTT [HYPO 779 g/kg wet wt (SD 15) vs. RE 780 g/kg wet wt (SD 20); time P 0.011; trial time P 0.055]. Resting energy expenditure was similar between hydration states (stable between 1.21 and 5.94 kJ·kg
1
·day
1
; trial P 0.904). Overall, despite acute mild hypohydration increasing plasma copeptin concentrations and decreasing fasted cell volume and muscle water, we found no effect on glycemic regulation.

Department/s

  • Cardiovascular Research - Hypertension
  • EXODIAB: Excellence in Diabetes Research in Sweden
  • EpiHealth: Epidemiology for Health

Publishing year

2019

Language

English

Pages

422-430

Publication/Series

Journal of Applied Physiology

Volume

126

Issue

2

Document type

Journal article

Publisher

American Physiological Society

Topic

  • Physiology

Status

Published

Research group

  • Cardiovascular Research - Hypertension

ISBN/ISSN/Other

  • ISSN: 8750-7587