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Olle Melander

Principal investigator

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Genome-wide interaction study of smoking behavior and non-small cell lung cancer risk in Caucasian population


  • Yafang Li
  • Xiangjun Xiao
  • Younghun Han
  • Olga Gorlova
  • David Qian
  • Natasha Leighl
  • Jakob S. Johansen
  • Matt Barnett
  • Chu Chen
  • Gary Goodman
  • Angela Cox
  • Fiona Taylor
  • Penella Woll
  • H. Erich Wichmann
  • Judith Manz
  • Thomas Muley
  • Angela Risch
  • Albert Rosenberger
  • Susanne M. Arnold
  • Eric B. Haura
  • Ciprian Bolca
  • Ivana Holcatova
  • Vladimir Janout
  • Milica Kontic
  • Jolanta Lissowska
  • Anush Mukeria
  • Simona Ognjanovic
  • Tadeusz M. Orlowski
  • Ghislaine Scelo
  • Beata Swiatkowska
  • David Zaridze
  • Per Bakke
  • Vidar Skaug
  • Shanbeh Zienolddiny
  • Eric J. Duell
  • Lesley M. Butler
  • Richard Houlston
  • María Soler Artigas
  • Kjell Grankvist
  • Mikael Johansson
  • Frances A. Shepherd
  • Michael W. Marcus
  • Hans Brunnström
  • Jonas Manjer
  • Olle Melander
  • David C. Muller
  • Kim Overvad
  • Antonia Trichopoulou
  • Rosario Tumino
  • Geoffrey Liu
  • Stig E. Bojesen
  • Xifeng Wu
  • Loic Le Marchand
  • Demetrios Albanes
  • Heike Bickeböller
  • Melinda C. Aldrich
  • William S. Bush
  • Adonina Tardon
  • Gad Rennert
  • M. Dawn Teare
  • John K. Field
  • Lambertus A. Kiemeney
  • Philip Lazarus
  • Aage Haugen
  • Stephen Lam
  • Matthew B. Schabath
  • Angeline S. Andrew
  • Pier Alberto Bertazzi
  • Angela C. Pesatori
  • David C. Christiani
  • Neil Caporaso
  • Mattias Johansson
  • James D. McKay
  • Paul Brennan
  • Rayjean J. Hung
  • Christopher I. Amos

Summary, in English

Non-small cell lung cancer is the most common type of lung cancer. Both environmental and genetic risk factors contribute to lung carcinogenesis. We conducted a genome-wide interaction analysis between single nucleotide polymorphisms (SNPs) and smoking status (never- versus ever-smokers) in a European-descent population. We adopted a two-step analysis strategy in the discovery stage: we first conducted a case-only interaction analysis to assess the relationship between SNPs and smoking behavior using 13 336 non-small cell lung cancer cases. Candidate SNPs with P-value <0.001 were further analyzed using a standard case-control interaction analysis including 13 970 controls. The significant SNPs with P-value <3.5 × 10-5 (correcting for multiple tests) from the case-control analysis in the discovery stage were further validated using an independent replication dataset comprising 5377 controls and 3054 non-small cell lung cancer cases. We further stratified the analysis by histological subtypes. Two novel SNPs, rs6441286 and rs17723637, were identified for overall lung cancer risk. The interaction odds ratio and meta-analysis P-value for these two SNPs were 1.24 with 6.96 × 10-7 and 1.37 with 3.49 × 10-7, respectively. In addition, interaction of smoking with rs4751674 was identified in squamous cell lung carcinoma with an odds ratio of 0.58 and P-value of 8.12 × 10-7. This study is by far the largest genome-wide SNP-smoking interaction analysis reported for lung cancer. The three identified novel SNPs provide potential candidate biomarkers for lung cancer risk screening and intervention. The results from our study reinforce that gene-smoking interactions play important roles in the etiology of lung cancer and account for part of the missing heritability of this disease.


  • EpiHealth: Epidemiology for Health
  • BioCARE: Biomarkers in Cancer Medicine improving Health Care, Education and Innovation
  • Department of Clinical Sciences, Lund
  • EXODIAB: Excellence in Diabetes Research in Sweden

Publishing year












Document type

Journal article


Oxford University Press


  • Cancer and Oncology
  • Medical Genetics




  • ISSN: 0143-3334