Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here:

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Olle Melander

Principal investigator

Default user image.

Cellular dehydration acutely degrades mood mainly in women : A counterbalanced, crossover trial


  • Hyun Gyu Suh
  • Harris R. Lieberman
  • Lisa T. Jansen
  • Abigail T. Colburn
  • J. D. Adams
  • Adam D. Seal
  • Cory L. Butts
  • Tracie M. Kirkland
  • Olle Melander
  • Tiphaine Vanhaecke
  • Alberto Dolci
  • Guillaume Lemetais
  • Erica T. Perrier
  • Stavros A. Kavouras

Summary, in English

It is unclear if mild-to-moderate dehydration independently affects mood without confounders like heat exposure or exercise. This study examined the acute effect of cellular dehydration on mood. Forty-nine adults (55 % female, age 39 (SD 8) years) were assigned to counterbalanced, crossover trials. Intracellular dehydration was induced with 2-h (0·1 ml/kg per min) 3 % hypertonic saline (HYPER) infusion or 0·9 % isotonic saline (ISO) as a control. Plasma osmolality increased in HYPER (pre 285 (SD 3), post 305 (SD 4) mmol/kg; P < 0·05) but remained unchanged in ISO (pre 285 (SD 3), post 288 (SD 3) mmol/kg; P > 0·05). Mood was assessed with the short version of the Profile of Mood States Questionnaire (POMS). The POMS sub-scale (confusion-bewilderment, depression-dejection, fatigue-inertia) increased in HYPER compared with ISO (P < 0·05). Total mood disturbance score (TMD) assessed by POMS increased from 10·3 (SD 0·9) to 16·6 (SD 1·7) in HYPER (P < 0·01), but not in ISO (P > 0·05). When TMD was stratified by sex, the increase in the HYPER trial was significant in females (P < 0·01) but not in males (P > 0·05). Following infusion, thirst and copeptin (surrogate for vasopressin) were also higher in females than in males (21·3 (SD 2·0), 14·1 (SD 1·4) pmol/l; P < 0·01) during HYPER. In conclusion, cellular dehydration acutely degraded specific aspects of mood mainly in women. The mechanisms underlying sex differences may be related to elevated thirst and vasopressin.


  • Cardiovascular Research - Hypertension
  • EpiHealth: Epidemiology for Health
  • EXODIAB: Excellence in Diabetes Research in Sweden

Publishing year







British Journal of Nutrition





Document type

Journal article


Cambridge University Press


  • Nutrition and Dietetics


  • Copeptin
  • Osmotic stimulation
  • Thirst
  • Underhydation
  • Vasopressin



Research group

  • Cardiovascular Research - Hypertension


  • ISSN: 0007-1145