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Ola Hansson

Ola Hansson

Principal investigator

Ola Hansson

MicroRNA‐100 Reduced Fetal Bovine Muscle Satellite Cell Myogenesis and Augmented Intramuscular Lipid Deposition by Modulating IGF1R

Author

  • Bilal Ahmad Mir
  • Elke Albrecht
  • Asghar Ali
  • Ola Hansson
  • Steffen Maak

Summary, in English

Previously, microRNA‐100 (miR‐100) and its putative mRNA target, insulin‐like growth factor receptor‐1 (IGF1R) were identified as differentially and inversely expressed in bovine longissimus dorsi (LD) muscles with divergent intramuscular fat (IMF) content by our group. While IGF1R signaling is implicated in myogenesis and muscle lipid metabolism, the underlying regulatory mechanisms are poorly understood. In the present study, we aimed to investigate the regulation of IGF1R by miR‐100 during bovine muscle satellite cell (BMSC) myogenesis and lipid deposition. MiR‐100 was confirmed to target the IGF1R 3′‐untranslated region (3′‐UTR) by luciferase reporter assay. Furthermore, expression of miR‐100 and IGF1R was reciprocal during BMSC differentiation, suggesting a crosstalk between the two. Correspondingly, miR‐100 mimic (agomiR) suppressed the levels of IGF1R, PI3K/AKT pathway signaling, myogenic gene MYOG, muscle structural components MYH7 and MYH8, whereas the inhibitor (antagomiR) had no clear stimulating effects. The IGF1R inhibitor (BMS‐754807) curtailed receptor levels and triggered atrophy in muscle myotubes but did not influence miR‐100 expression. AgomiR increased oleic acid‐induced lipid deposition in BMSC myotubes supporting its involvement in intramuscular fat deposition, while antagomiR had no effect. Moreover, mitochondrial beta‐oxidation and long‐chain fatty acid synthesis‐related genes were modulated by agomiR addition. Our results demonstrate modulatory roles of miR‐100 in BMSC development, lipid deposition, and metabolism and suggest a role of miR‐100 in marbling characteristics of meat animals and fat oxidation in muscle.

Department/s

  • Genomics, Diabetes and Endocrinology
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2022

Language

English

Publication/Series

Cells

Volume

11

Issue

3

Document type

Journal article

Publisher

MDPI AG

Topic

  • Endocrinology and Diabetes
  • Physiology
  • Cell and Molecular Biology

Keywords

  • Embryonic bovine muscle satellite cells
  • Fatty acids
  • IGF1R
  • Intramuscular fat
  • Marbling
  • MiRNAs

Status

Published

Research group

  • Genomics, Diabetes and Endocrinology

ISBN/ISSN/Other

  • ISSN: 2073-4409