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Nils Wierup

Nils Wierup

Senior lecturer

Nils Wierup

CART-peptide immunoreactivity in enteric nerves in patients with Hirschsprung's disease

Author

  • Anna Gunnarsdottir
  • Nils Wierup
  • Lars Torsten Larsson
  • M. J. Kuhar
  • Eva Ekblad

Summary, in English

Aims: Cocaine- and amphetamine-regulated transcript (CART)-peptide is found in the brain and participates in the control of feeding behavior. It is also expressed in the peripheral nervous system and is suggested to have neuromodulatory and/or neurotrophic effects in rat intestine. The aims of this study were to investigate the presence of CART-peptide in the normal ganglionic as well as aganglionic intestine from patients with Hirschsprung's disease and the pepticle's possible coexistence with other neurotransmitters. Methods: Intestinal specimens from nine patients with Hirschsprung's disease were examined using immunohistochemistry. A double immunostaining technique was used in order to elucidate the presence of CART-peptide in NOS and VIP-containing enteric neurons. Results: in ganglionic intestine, CART-peptide was found in numerous nerve fibers, predominantly within the smooth muscle layers and in myenteric nerve cell bodies. A high degree of co-localization of CART with NOS and VIP was seen. Only very few CART immunoreactive nerve fibers and no nerve cell bodies were found in the aganglionic intestine. Conclusions: This is the first report on the presence of CART-peptide in the human intestine. In the ganglionic intestine CART was detected mainly in myenteric neurons, while only very few CART-IR nerve fibers were found in the aganglionic intestine. This, together with the coexistence of CART with NOS and VIP, indicates an intrinsic origin of the CART-containing neurons and suggests that CART may influence NO and VIP-induced effects.

Department/s

  • Neurogastroenterology
  • Paediatrics (Lund)
  • Department of Experimental Medical Science

Publishing year

2007

Language

English

Pages

184-189

Publication/Series

European Journal of Pediatric Surgery

Volume

17

Issue

3

Document type

Journal article

Publisher

Georg Thieme Verlag KG

Topic

  • Pediatrics

Keywords

  • Hirschsprung's disease
  • gut motility
  • intestine
  • CART
  • neuropeptide
  • ENS

Status

Published

Research group

  • Neurogastroenterology

ISBN/ISSN/Other

  • ISSN: 1439-359X