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Nils Wierup

Nils Wierup

Senior lecturer

Nils Wierup

Antral G-cell in gastrin and gastrin-cholecystokinin knockout animals

Author

  • L Friis-Hansen
  • Nils Wierup
  • J F Rehfeld
  • Frank Sundler

Summary, in English

The antral hormone gastrin is the key regulator of gastric acid secretion, mucosal growth and differentiation. Gastrin is synthesized in the endocrine G-cells in the antroduodenal mucosa. We have now examined the way in which the loss of gastrin alone or gastrin plus cholecystokinin (CCK) affects the antral G-cell. Immunohistochemistry, radioimmunoassay and quantitative real-time polymerase chain reaction techniques were employed to examine the expression of genes belonging to the G-cell secretory pathway in gastrin and gastrin-CCK knockout mice. Transmission electron microscopy was used to examine the ultrastructure of the G-cells. The number of G-cells increased but the secretory granules were few and abnormally small in the G-cells of both mouse models compared with wildtypes. Thus, gastrin is not necessary for the formation of G-cells as such but the lack of gastrin reduces the number and size of their secretory granules suggesting that gastrin is vital for the formation and/or maintenance of secretory granules in G-cells.

Department/s

  • Department of Experimental Medical Science

Publishing year

2005

Language

English

Pages

141-146

Publication/Series

Cell and Tissue Research

Volume

321

Issue

1

Document type

Journal article

Publisher

Springer

Topic

  • Cell Biology

Keywords

  • transgenic mice
  • ultrastructure
  • G-cells
  • secretion
  • gastrin
  • cholecystokinin

Status

Published

ISBN/ISSN/Other

  • ISSN: 1432-0878