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Photo: KG Pressfoto

Marju Orho-Melander

Professor

Photo: KG Pressfoto

Polymorphism in the Calpain 10 gene influences glucose metabolism in human fat cells

Author

  • J Hoffstedt
  • M Ryden
  • P Lofgren
  • Marju Orho-Melander
  • Leif Groop
  • P Arner

Summary, in English

Aims/hypothesis. A common G to A polymorphism (UCSNP-43) in the Calpain 10 gene was recently found to be associated with Type 11 (non-insulin-dependent) diabetes mellitus and variations in post-absorptive and insulin stimulated glucose metabolism in vivo. We aimed to study the influence of Calpain 10 polymorphism on insulin action in fat cells. Methods. Calpain 10 polymorphism (UCSNP-19, -43 or -63) were set in relation to lipolysis and lipogenesis in isolated subcutaneous adipocytes of 46 apparently healthy non-obese subjects. Results. For UCSNP-43 the GIG genotype had twofold higher basal and insulin stimulated rates as compared with AA/AG genotypes. However, there was no genotype effect on basal or insulin inhibited lipolysis rates in fat cells. The protein amount of GLUT 4 in adipocytes was not influenced by the polymorphism. Fat cells expressed mRNA for the Calpain 10 gene at a relatively high concentration, about 4 amol/mug RNA, which is similar to that of uncoupling protein-2. Neither a UCSNP-19 nor a UCSNP-63 polymorphism in the Calpain 10 gene was found to be associated with basal or insulin-induced adipocyte lipolysis and lipogenesis. None of the polymorphisms influenced body mass index or fasting plasma concentrations of insulin and glucose in 693 non-obese healthy subjects. Conclusion/interpretation. The Calpain 10 gene could be involved in the regulation of glucose metabolism but not lipolysis in human fat cells, although it does not involve adipocyte GLUT-4 protein content. It is possible that the Calpain 10 gene predisposes to diabetes by influencing the glucose metabolism.

Department/s

  • Genomics, Diabetes and Endocrinology

Publishing year

2002

Language

English

Pages

276-282

Publication/Series

Diabetologia

Volume

45

Issue

2

Document type

Journal article

Publisher

Springer

Topic

  • Endocrinology and Diabetes

Keywords

  • insulin
  • lipogenesis
  • lipolysis
  • polymerase chain reaction
  • mRNA
  • adipose tissue
  • GLUT-4

Status

Published

Research group

  • Genomics, Diabetes and Endocrinology

ISBN/ISSN/Other

  • ISSN: 1432-0428