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Maria Gomez

Maria Gomez

Professor

Maria Gomez

Long-term regulation of contractility and calcium current in smooth muscle

Author

  • Maria Gomez
  • Karl Swärd

Summary, in English

Longitudinal smooth muscle strips from guinea pig ileum were cultured in vitro for 5 days, and the relationship between extracellular Ca2+ and force in high-K+ medium was evaluated. In strips cultured with 10% fetal calf serum (FCS), this relationship was shifted to the right (50% effective concentration changed by 2-3 mM) compared with strips cultured without FCS. The shift was prevented by inclusion of verapamil (1 microM) during culture and mimicked by ionomycin in the absence of FCS. The intracellular Ca2+ concentration ([Ca2+]i) during stimulation with high-K+ solution or carbachol was reduced after culture with FCS, whereas the [Ca2+]i-force relationship was unaffected. Cells were isolated from cultured strips, and whole cell voltage-clamp experiments were performed. Maximum inward Ca2+ current (10 mM Ba2+), normalized to cell capacitance, was almost three times smaller in cells isolated from strips cultured with FCS. Culture with 1 microM verapamil prevented this reduction. These results suggest that increased [Ca2+]i during culture downregulates Ca2+ current density, with associated effects on contractility.

Department/s

  • Vascular Physiology

Publishing year

1997

Language

English

Pages

1714-1720

Publication/Series

American Journal of Physiology: Cell Physiology

Volume

273

Issue

5

Document type

Journal article

Publisher

American Physiological Society

Topic

  • Physiology

Keywords

  • fura 2
  • verapamil
  • patch clamp
  • ionomycin
  • tissue culture

Status

Published

Research group

  • Vascular Physiology

ISBN/ISSN/Other

  • ISSN: 1522-1563