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Maria Gomez

Maria Gomez

Professor

Maria Gomez

Effects of polyamines on voltage-activated calcium channels in guinea-pig intestinal smooth muscle

Author

  • Maria Gomez
  • Per Hellstrand

Summary, in English

Effects of polyamines on the spontaneous mechanical and electrical activity of guinea-pig intestinal smooth muscle were studied. Spermine and spermidine inhibited action potential generation and contractions, while putrescine had no effect. Single smooth muscle cells were isolated from the longitudinal muscle layer of the guinea-pig ileum. Whole-cell voltage-clamp experiments were carried out to investigate the effects of polyamines on current through voltage-activated Ca2+ channels. Spermine and spermidine (0.1-1 mM) reduced the inward current in a concentration-dependent manner. Spermine blocked current activated by the dihydropyridine agonist BAY K 8644 (1 microM), whereas no additional inhibition by spermine was seen after blockage of dihydropyridine-sensitive channels by nifedipine (0.1 microM). Inhibition by spermine or spermidine did not shift the peak of the current voltage relation of the inward current. Steady-state activation and inactivation relationships were not affected and thus the amplitude, but not the voltage dependence, of the window current responsible for Ca2+ inflow during sustained depolarization was affected. Putrescine (1 mM) had no significant effect on the inward current. These results suggest that spermine and spermidine inhibit contraction in spontaneously active intestinal smooth muscle by inhibiting Ca2+ current responsible for generation of action potentials.

Department/s

  • Vascular Physiology

Publishing year

1995

Language

English

Pages

501-507

Publication/Series

Pflügers Archiv

Volume

430

Issue

4

Document type

Journal article

Publisher

Springer

Topic

  • Physiology

Keywords

  • Intestinal smooth muscle
  • Whole-cell voltage clamp
  • Ca2+ channels
  • Polyamines
  • Spermine
  • Spermidine
  • Putrescine

Status

Published

Research group

  • Vascular Physiology

ISBN/ISSN/Other

  • ISSN: 0031-6768