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Maria Gomez

Maria Gomez

Professor

Maria Gomez

The importance of fibroblasts in remodelling of the human uterine cervix during pregnancy and parturition.

Author

  • Erik Malmström
  • Maria Sennstrom
  • Anna Holmberg
  • Helena Frielingsdorf
  • Erik Eklund
  • Lars Malmstrom
  • Ellen Tufvesson
  • Maria Gomez
  • Gunilla Westergren-Thorsson
  • Gunvor Ekman-Ordeberg
  • Anders Malmstrom

Summary, in English

It is well established that fibroblasts play a crucial role in pathophysiological extracellular matrix remodelling. The aim of this project is to elucidate their role in normal physiological remodelling. Specifically, the remodelling of the human cervix during pregnancy, resulting in an enabled passage of the child, is used as the model system. Fibroblast cultures were established from cervices of non-pregnant women, women after 36 weeks of pregnancy and women directly after partus. The cells were immunostained and quantified by western blots for differentiation markers. The cultures were screened for cytokine and metalloproteinase production and characterized by global proteome analysis. The cell cultures established from partal donors differ significantly from those from non-pregnant donors, which is in accordance with in vivo findings. A decrease in alpha-smooth actin and prolyl-4hydroxylase and an increase in interleukin (IL)-6, IL-8 and matrix metalloproteinases (MMP)-1 and MMP-3 were observed in cultures from partal donors. 2D-gel electrophoresis followed by mass spectrometry showed that the expression of 59 proteins was changed significantly in cultures of partal donors. The regulated proteins are involved in protein kinase C signalling, Ca2+ binding, cytoskeletal organization, angiogenesis and degradation. Our data suggest that remodelling of the human cervix is orchestrated by fibroblasts, which are activated or recruited by the inflammatory processes occurring during the ripening cascade.

Department/s

  • Infection Medicine (BMC)
  • Department of Experimental Medical Science
  • Paediatrics (Lund)
  • Lung physiology and biomarkers
  • Respiratory Medicine and Allergology
  • Cardiovascular Research - Immunity and Atherosclerosis
  • Lung Biology

Publishing year

2007

Language

English

Pages

333-341

Publication/Series

Molecular Human Reproduction

Volume

13

Document type

Journal article

Publisher

Oxford University Press

Topic

  • Obstetrics, Gynecology and Reproductive Medicine

Status

Published

Research group

  • Lung physiology and biomarkers
  • Cardiovascular Research - Immunity and Atherosclerosis
  • Lung Biology

ISBN/ISSN/Other

  • ISSN: 1460-2407