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Luis Sarmiento-Pérez

Assistant researcher

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Valoración de una novedosa tecnología para la detección de enterovirus en aguas negras

Evaluation of a new technology for the detection of enterovirus in sewage

Author

  • L Sarmiento Pérez
  • P Más Lago
  • I Avalos Redón
  • R Palomera Puentes
  • J Barrios Olivera
  • M Bello Corredor

Summary, in English

This paper reported the appraisal of a novel technology for the detection of enterovirus in sewage based on a viral recovery method using polyethylenglycol as a concentrating agent and on the combined use of viral isolation and polymerase chain reaction as viral detection and identification techniques. It was also confirmed that the viral recovery method is highly efficient since it allows to recover all viruses present in sewage and to preserve their infective capacity. It was proved that the polymerase chain reaction (PCR) is less sensitive than cell culture for detecting enteroviruses in sewage. It is also possible to use guadinine isothiocyanate, whose components can be prepared in the lab, as an alternative method for taking out and purifying nucleic acids instead of using the conventional TRIZOL method which is the one recommended in these cases by the World Health Organization.

Publishing year

2000-07-11

Language

Spanish

Pages

71-166

Publication/Series

Revista cubana de medicina tropical

Volume

51

Issue

3

Document type

Journal article

Publisher

Editorial Ciencias Medicas

Topic

  • Microbiology in the medical area

Keywords

  • Enterovirus/isolation & purification
  • Evaluation Studies as Topic
  • Sewage/virology
  • Virology/methods

Status

Published

ISBN/ISSN/Other

  • ISSN: 0375-0760