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Luis Sarmiento-Pérez

Assistant researcher

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Caracterización intratípica de Poliovirus por la técnica de reacción en cadena de la polimerasa

The intratypic characterization of the poliovirus by the polymerase chain reaction technic

Author

  • I Avalos Redón
  • P J Más Lago
  • L R Sarmiento Pérez
  • R Palomera Puente
  • M Muné Jiménez
  • M Bello Corredor
  • L Pérez Santos

Summary, in English

The polymerase chain reaction techniques was introduced for the intratypic characterization of Poliovirus. Primers were used only to promote the amplification of the Sabin vaccine strains proved by electrophoretic run of the amplified DNA products (Sabin 1-97 pb, Sabin 2-71 pb, Sabin 3-44 pb) and whose specificity was satisfactorily verified. 23 Cuban poliovirus strains isolated and identified at the Laboratory of Enterovirus of the "Pedro Kourí" Tropical Medicine Institute from 1993 to 1994 were studied by this technique. All of them were of the vaccine type. It was observed how the Sabin vaccine poliovirus may be the cause of viral meningoencephalitis as a milder neurological complication. This study provided one more evidence about the non circulation of the wild poliovirus in Cuba.

Publishing year

1998

Language

Spanish

Pages

4-100

Publication/Series

Revista cubana de medicina tropical

Volume

50

Issue

2

Document type

Journal article

Publisher

Editorial Ciencias Medicas

Topic

  • Microbiology in the medical area

Keywords

  • Base Sequence
  • Humans
  • Molecular Sequence Data
  • Oligonucleotide Probes
  • Poliovirus/classification
  • Polymerase Chain Reaction/methods
  • RNA, Viral/genetics

Status

Published

ISBN/ISSN/Other

  • ISSN: 0375-0760