Your browser has javascript turned off or blocked. This will lead to some parts of our website to not work properly or at all. Turn on javascript for best performance.

The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Default user image.

Luis Sarmiento-Pérez

Assistant researcher

Default user image.

Analysis of respiratory syncytial virus in clinical samples by reverse transcriptase-polymerase chain reaction restriction mapping

Author

  • A Valdivia
  • C Savón
  • D Chacón
  • L Sarmiento Pérez
  • L Morier
  • A Otero
  • Y Soto
  • S Oropesa
  • A Goyenechea

Summary, in English

The aim of this study was to develop a polymerase chain reaction (PCR) for the detection of respiratory syncytial virus (RSV) genomes. The primers were designed from published sequences and selected from conserved regions of the genome encoding for the N protein of subgroups A and B of RSV. PCR was applied to 20 specimens from children admitted to the respiratory ward of "William Soler" Pediatric Hospital in Havana City with a clinical diagnosis of bronchiolitis. The PCR was compared with viral isolation and with an indirect immunofluorescence technique that employs monoclonal antibodies of subgroups A and B. Of 20 nasopharyngeal exudates, 10 were found positive by the three assayed methods. In only two cases, samples that yielded positive RNA-PCR were found negative by indirect immunofluorescence and cell culture. Considering viral isolation as the "gold standard" technique, RNA-PCR had 100% sensitivity and 80% specificity. RNA-PCR is a specific and sensitive technique for the detection of the RSV genome. Technical advantages are discussed.

Publishing year

1997-05-01

Language

English

Pages

93-389

Publication/Series

Memorias do Instituto Oswaldo Cruz

Volume

92

Issue

3

Document type

Journal article

Publisher

Fundacao Oswaldo Cruz

Topic

  • Microbiology in the medical area

Keywords

  • Humans
  • Polymerase Chain Reaction
  • RNA-Directed DNA Polymerase
  • Respiratory Syncytial Virus Infections/diagnosis
  • Respiratory Syncytial Virus, Human/genetics
  • Restriction Mapping

Status

Published

ISBN/ISSN/Other

  • ISSN: 0074-0276