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Luis Sarmiento-Pérez

Assistant researcher

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Occurrence of enterovirus RNA in serum of children with newly diagnosed type 1 diabetes and islet cell autoantibody-positive subjects in a population with a low incidence of type 1 diabetes

Author

  • Luis Sarmiento
  • Eduardo Cabrera-Rode
  • Lister Lekuleni
  • Ileana Cuba
  • Gisela Molina
  • Magile Fonseca
  • Lai Heng-Hung
  • Abel Diaz Borroto
  • Pedro Gonzalez
  • Pedro Mas-Lago
  • Oscar Diaz-Horta

Summary, in English

BACKGROUND: The penetrance of type 1 diabetes mellitus (T1DM) in a genetically susceptible population is largely determined by environmental influences amongst which the human enteroviruses are prominent putative factors.

AIM/HYPOTHESIS: The aim of this study was to determine the occurrence of enterovirus RNA in serum of children with type 1 diabetes at onset and ICA-positive subjects in a population with low incidence of type 1 diabetes and high circulation of enteroviruses.

SUBJECTS AND METHODS: Serum samples were collected from children with newly diagnosed type 1 diabetes (n = 34); islet autoantibody-positive (n = 32) and -negative (n = 31) first-degree relatives of type 1 diabetic patients; and control subjects (n = 194). Enteroviral RNA was assessed using a highly sensitive reverse transcriptase-polymerase chain reaction method.

RESULTS: The frequency of positive signals corresponding to enteroviral sequence amplifications were higher in newly diagnosed T1DM children (9/34, 26.5%) and islet autoantibody-positive first-degree relatives (5/32, 15.6%) than in their corresponding matched controls (2/68, 2.9%, p = 0.0007 and 0/64, 0.0%, p = 0.0033, respectively). The presence of enteroviral RNA appeared to be associated with severe diabetic ketoacidosis at onset (pH < 7.1, p = 0.0328) and high ICA titres ( > or = 20 JDF units, p < 0.05).

CONCLUSIONS: Despite there is a high circulation of enteroviruses and a low type 1 diabetes incidence in the Cuban population, the presence of enteroviral RNA is associated with type 1 diabetes and beta-cell autoimmunity and is similar to European countries in which this scenario is reversed.

Publishing year

2007-11

Language

English

Pages

5-540

Publication/Series

Autoimmunity

Volume

40

Issue

7

Document type

Journal article

Publisher

Taylor & Francis

Topic

  • Endocrinology and Diabetes

Keywords

  • Adolescent
  • Autoantibodies/blood
  • Child
  • Child, Preschool
  • Cuba
  • Diabetes Mellitus, Type 1/blood
  • Diabetic Ketoacidosis/blood
  • Enterovirus
  • Enterovirus Infections/blood
  • Europe
  • Female
  • Humans
  • Incidence
  • Infant
  • Insulin-Secreting Cells/immunology
  • Male
  • RNA, Viral/blood

Status

Published

ISBN/ISSN/Other

  • ISSN: 0891-6934