Detección de poliovirus en Aguas residuales de Armenia, Colombia
Detecting poliovirus in wastewater from Armenia, Colombia
Summary, in English
OBJECTIVES: Implementing environmental surveillance for poliovirus circulating from wastewater.
METHODS: Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers.
RESULTS: It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country.
CONCLUSION: Wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.
Revista de Salud Publica
Universidad Nacional de Colombia
- Microbiology in the medical area
- Environmental Microbiology
- Poliovirus Vaccine, Oral
- Polyethylene Glycols
- RNA, Viral/analysis
- Reverse Transcriptase Polymerase Chain Reaction
- Sodium Chloride
- ISSN: 0124-0064