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Luis Sarmiento-Pérez

Assistant researcher

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Detección de poliovirus en Aguas residuales de Armenia, Colombia

Detecting poliovirus in wastewater from Armenia, Colombia

Author

  • Maria M González
  • Luis Sarmiento
  • Jhon Carlos Castaño
  • Alejandra M Giraldo
  • Alexander Salazar
  • Nini J Muñoz

Summary, in English

OBJECTIVES: Implementing environmental surveillance for poliovirus circulating from wastewater.

METHODS: Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers.

RESULTS: It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country.

CONCLUSION: Wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.

Publishing year

2006-05

Language

Spanish

Pages

13-23

Publication/Series

Revista de Salud Publica

Volume

8

Issue

Suppl 1

Document type

Journal article

Publisher

Universidad Nacional de Colombia

Topic

  • Microbiology in the medical area

Keywords

  • Colombia
  • Environmental Microbiology
  • Poliovirus/classification
  • Poliovirus Vaccine, Oral
  • Polyethylene Glycols
  • RNA, Viral/analysis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sewage/virology
  • Sodium Chloride
  • Virology/methods

Status

Published

ISBN/ISSN/Other

  • ISSN: 0124-0064