
Luis Sarmiento-Pérez
Assistant researcher

Detección rápida de Enterovirus mediante un método directo de reacción en cadena de la polimerasa
Rapid detection of Enterovirus by a direct method of polymerase chain reaction
Author
Summary, in English
For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of RNA extraction and purification prior to the reaction does not affect the susceptibility and specificity of the system, in the rapid detection of Enterovirus genomes and identification of vaccinal strains of poliovirus.
Publishing year
2000-12-07
Language
Spanish
Pages
15-20
Publication/Series
Revista cubana de medicina tropical
Volume
52
Issue
1
Links
Document type
Journal article
Publisher
Editorial Ciencias Medicas
Topic
- Microbiology in the medical area
Keywords
- Base Sequence
- DNA, Viral/genetics
- Enterovirus/genetics
- Enterovirus Infections/diagnosis
- Humans
- Meningoencephalitis/diagnosis
- Molecular Sequence Data
- Polymerase Chain Reaction/methods
- RNA, Viral/genetics
- Sensitivity and Specificity
Status
Published
ISBN/ISSN/Other
- ISSN: 0375-0760