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Luis Sarmiento-Pérez

Assistant researcher

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Detección rápida de Enterovirus mediante un método directo de reacción en cadena de la polimerasa

Rapid detection of Enterovirus by a direct method of polymerase chain reaction

Author

  • L Sarmiento Pérez
  • I Avalos Redón
  • Y Ramos Valdés
  • P Más Lago
  • M Bello Corredor
  • R Palomera Puentes
  • J Barrios Olivera

Summary, in English

For the detection of Enterovirus, we devised a direct economical method of polymerase chain reaction which does not require a previous extraction of ribonucleic acid and uses infected cell culture supernatants. The system was developed on the basis of universal primers of Enterovirus and specific primers of vaccinal strain Sabin 1. The achieved results proved that the non-existence of methods of RNA extraction and purification prior to the reaction does not affect the susceptibility and specificity of the system, in the rapid detection of Enterovirus genomes and identification of vaccinal strains of poliovirus.

Publishing year

2000-12-07

Language

Spanish

Pages

15-20

Publication/Series

Revista cubana de medicina tropical

Volume

52

Issue

1

Document type

Journal article

Publisher

Editorial Ciencias Medicas

Topic

  • Microbiology in the medical area

Keywords

  • Base Sequence
  • DNA, Viral/genetics
  • Enterovirus/genetics
  • Enterovirus Infections/diagnosis
  • Humans
  • Meningoencephalitis/diagnosis
  • Molecular Sequence Data
  • Polymerase Chain Reaction/methods
  • RNA, Viral/genetics
  • Sensitivity and Specificity

Status

Published

ISBN/ISSN/Other

  • ISSN: 0375-0760