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ludc web

Lena Eliasson

Principal investigator

ludc web

Electrophysiology of pancreatic beta-cells in intact mouse islets of Langerhans

Author

  • Patrik Rorsman
  • Lena Eliasson
  • Takahiro Kanno
  • Quan Zhang
  • Sven Gopel

Summary, in English

When exposed to intermediate glucose concentrations (6-16 mol/l), pancreatic beta-cells in intact islets generate bursts of action potentials (superimposed on depolarised plateaux) separated by repolarised electrically silent intervals. First described more than 40 years ago, these oscillations have continued to intrigue beta-cell electrophysiologists. To date, most studies of beta-cell ion channels have been performed on isolated cells maintained in tissue culture (that do not burst). Here we will review the electrophysiological properties of beta-cells in intact, freshly isolated, mouse pancreatic islets. We will consider the role of ATP-regulated K+-channels (K-ATP-channels), small-conductance Ca2+-activated K+-channels and voltage-gated Ca2+-channels in the generation of the bursts. Our data indicate that K-ATP-channels not only constitute the glucose-regulated resting conductance in the beta-cell but also provide a variable K+- conductance that influence the duration of the bursts of action potentials and the silent intervals. We show that inactivation of the voltage-gated Ca2+-current is negligible at voltages corresponding to the plateau potential and consequently unlikely to play a major role in the termination of the burst. Finally, we propose a model for glucose-induced beta-cell electrical activity based on observations made in intact pancreatic islets. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.

Department/s

  • Diabetes - Islet Cell Exocytosis
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2011

Language

English

Pages

224-235

Publication/Series

Progress in Biophysics and Molecular Biology

Volume

107

Issue

2

Document type

Journal article review

Publisher

Elsevier

Topic

  • Endocrinology and Diabetes

Keywords

  • Pancreas
  • Beta-cell
  • Insulin
  • Ion channels
  • Glucose
  • Electrical activity

Status

Published

Research group

  • Diabetes - Islet Cell Exocytosis

ISBN/ISSN/Other

  • ISSN: 1873-1732