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ludc web

Lena Eliasson

Principal investigator

ludc web

SUR1 Regulates PKA-independent cAMP-induced Granule Priming in Mouse Pancreatic B-cells.

Author

  • Lena Eliasson
  • Xiaosong Ma
  • Erik Renström
  • Sebastian Barg
  • Per-Olof Berggren
  • Juris Galvanovskis
  • Jesper Gromada
  • Xingjun Jing
  • Ingmar Lundquist
  • Albert Salehi
  • Sabine Sewing
  • Patrik Rorsman

Summary, in English

Measurements of membrane capacitance were applied to dissect the cellular mechanisms underlying PKA-dependent and -independent stimulation of insulin secretion by cyclic AMP. Whereas the PKA-independent (Rp-cAMPS–insensitive) component correlated with a rapid increase in membrane capacitance of ~80 fF that plateaued within ~200 ms, the PKA-dependent component became prominent during depolarizations >450 ms. The PKA-dependent and -independent components of cAMP-stimulated exocytosis differed with regard to cAMP concentration dependence; the Kd values were 6 and 29 µM for the PKA-dependent and -independent mechanisms, respectively. The ability of cAMP to elicit exocytosis independently of PKA activation was mimicked by the selective cAMP-GEFII agonist 8CPT-2Me-cAMP. Moreover, treatment of B-cells with antisense oligodeoxynucleotides against cAMP-GEFII resulted in partial (50%) suppression of PKA-independent exocytosis. Surprisingly, B-cells in islets isolated from SUR1-deficient mice (SUR1-/- mice) lacked the PKA-independent component of exocytosis. Measurements of insulin release in response to GLP-1 stimulation in isolated islets from SUR1-/- mice confirmed the complete loss of the PKA-independent component. This was not attributable to a reduced capacity of GLP-1 to elevate intracellular cAMP but instead associated with the inability of cAMP to stimulate influx of Cl- into the granules, a step important for granule priming. We conclude that the role of SUR1 in the B cell extends beyond being a subunit of the plasma membrane KATP-channel and that it also plays an unexpected but important role in the cAMP-dependent regulation of Ca2+-induced exocytosis.

Department/s

  • Diabetes - Islet Cell Exocytosis
  • Department of Experimental Medical Science
  • Department of Clinical Sciences, Malmö
  • Drug Target Discovery

Publishing year

2003

Language

English

Pages

181-197

Publication/Series

Journal of General Physiology

Volume

121

Issue

3

Document type

Journal article

Publisher

Rockefeller Institute for Medical Research

Topic

  • Endocrinology and Diabetes
  • Pharmacology and Toxicology

Status

Published

Research group

  • Diabetes - Islet Cell Exocytosis
  • Drug Target Discovery

ISBN/ISSN/Other

  • ISSN: 0022-1295