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ludc web

Lena Eliasson

Principal investigator

ludc web

MiR-184 regulates pancreatic β-cell function according to glucose metabolism.

Author

  • Sudhir G Tattikota
  • Thomas Rathjen
  • Jean Hausser
  • Aditya Khedkar
  • Uma D Kabra
  • Varun K Pandey
  • Matthias Sury
  • Hans-Hermann Wessels
  • Ines Mollet
  • Lena Eliasson
  • Matthias Selbach
  • Robert P Zinzen
  • Mihaela Zavolan
  • Sebastian Kadener
  • Matthias Tschöp
  • Martin Jastroch
  • Marc R Friedländer
  • Matthew N Poy

Summary, in English

In response to fasting or hyperglycemia, the pancreatic β-cell alters its output of secreted insulin; however the pathways governing this adaptive response are not entirely established. While the precise role of microRNAs (miRNAs) is also unclear, a recurring theme emphasizes their function in cellular stress responses. We recently showed that miR-184, an abundant miRNA in the β-cell, regulates compensatory proliferation and secretion during insulin resistance. Consistent with previous studies showing miR-184 suppresses insulin release, expression of this miRNA was increased in islets after fasting, demonstrating an active role in the β-cell as glucose levels lower and the insulin demand ceases. Additionally, miR-184 was negatively regulated upon administration of a sucrose-rich diet in Drosophila demonstrating strong conservation of this pathway through evolution. Furthermore, miR-184 and its target Argonaute2 (Ago2) remained inversely correlated as concentrations of extracellular glucose increased, underlining a functional relationship between this miRNA and its targets. Lastly, restoration of Ago2 in the presence of miR-184 rescued suppression of miR-375-targeted genes suggesting these genes act in a coordinated manner during changes in the metabolic context. Together, these results highlight the adaptive role of miR-184 according to glucose metabolism and suggest the regulatory role of this miRNA in energy homeostasis is highly conserved.

Department/s

  • Diabetes - Islet Cell Exocytosis
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2015

Language

English

Pages

20284-20294

Publication/Series

Journal of Biological Chemistry

Volume

290

Issue

33

Document type

Journal article

Publisher

ASBMB

Topic

  • Endocrinology and Diabetes

Status

Published

Research group

  • Diabetes - Islet Cell Exocytosis

ISBN/ISSN/Other

  • ISSN: 1083-351X