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ludc web

Lena Eliasson

Principal investigator

ludc web

Adenovirus-mediated silencing of Synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets

Author

  • M Iezzi
  • Lena Eliasson
  • M Fukuda
  • C B Wollheim

Summary, in English

Synaptotagmins (Syts) are involved in Ca2+-dependent insulin release. However, which Syt isoform is functional in primary beta-cells remains unknown. We demonstrate by electron microscopy of pancreatic islets, the association of Syt 9 with insulin granules. Silencing of Syt 9 by RNA interference adenovirus in islet cells had no effect on the expression of Syt 5, Syt 7 and Syt 3 isoforms. The latter was localized at the plasma membrane of pancreatic polypeptide cells. Insulin release in response to glucose or tolbutamide was strongly inhibited in Syt 9 deficient islets, whereas exocytosis potentiated by raising cAMP levels, was unaltered. Thus, Syt 9 may act as Ca2+ sensor for beta-cell secretion.

Department/s

  • Diabetes - Islet Cell Exocytosis

Publishing year

2005

Language

English

Pages

5241-5246

Publication/Series

FEBS Letters

Volume

579

Issue

23

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Biological Sciences

Keywords

  • insulin exocytosis
  • pancreatic islets
  • electron
  • RNA
  • microscopy
  • interference
  • beta-cell

Status

Published

Research group

  • Diabetes - Islet Cell Exocytosis

ISBN/ISSN/Other

  • ISSN: 1873-3468