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Leif Groop

Leif Groop

Principal investigator

Leif Groop

Continuous and simultaneous determination of venous blood metabolites

Author

  • Anneli Andersson
  • Qun Chen
  • Leif Groop
  • Leif Bülow
  • Bin Xie

Summary, in English

Metabolic syndrome is associated with cardiovascular disease, type 2 diabetes mellitus (T2DM) and prediabetes. Metabolic syndrome is a cluster of interrelated clinical disorders. Difficulties in regulating glucose levels in blood are implicated in many of these disorders. Lactate, another energy metabolite, is produced under anaerobic conditions and can be used to monitor the balance between aerobic and anaerobic metabolism. Tested together, these metabolite levels can provide pro-diagnostic information that improves patient outcomes. Glucose and lactate were determined continuously and simultaneously in whole blood using a dual-channel thermal biosensor device in which one channel employed glucose oxidase for glucose analysis in comparison with lactate oxidase for lactate analysis in the others. No detectable clogging or interference was observed using venous blood samples. The linear detection range for both the glucose and lactate assays was 0.5–45 mM. The sampling rate of up to 24 samples per hour with assay cycle time of 2.5 min was achieved. Comparative analysis between our device and the HemoCue method showed an excellent correlation. The device was stable for hundreds of injections over a period of 45 days. The broad linear range, fast response and detection sensitivity are satisfactory for the clinical requirements, e.g. for diabetic or cardiovascular patients in intensive care units or surgical operation, where the tight control of blood glucose can decrease morbidity or mortality.

Department/s

  • Pure and Applied Biochemistry
  • Department of Clinical Sciences, Lund
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2017-08-15

Language

English

Pages

270-274

Publication/Series

Talanta

Volume

171

Document type

Journal article

Publisher

Elsevier

Topic

  • Endocrinology and Diabetes
  • Biochemistry and Molecular Biology

Keywords

  • Continuous monitoring
  • Diabetes monitoring
  • Glucose
  • Lactate
  • Thermal biosensor
  • Whole blood analysis

Status

Published

ISBN/ISSN/Other

  • ISSN: 0039-9140