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Herzog

Katharina Herzog

Visiting research fellow

Herzog

An UPLC-MS/MS assay to measure glutathione as marker for oxidative stress in cultured cells

Author

  • Katharina Herzog
  • Lodewijk Ijlst
  • Arno G. van Cruchten
  • Carlo W.T. van Roermund
  • Wim Kulik
  • Ronald J.A. Wanders
  • Hans R. Waterham

Summary, in English

Oxidative stress plays a role in the onset and progression of a number of diseases, such as Alzheimer’s disease, diabetes and cancer, as well as ageing. Oxidative stress is caused by an increased production of reactive oxygen species and reduced antioxidant activity, resulting in the oxidation of glutathione. The ratio of reduced to oxidised glutathione is often used as a marker of the redox state in the cell. Whereas a variety of methods have been developed to measure glutathione in blood samples, methods to measure glutathione in cultured cells are scarce. Here we present a protocol to measure glutathione levels in cultured human and yeast cells using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS).

Department/s

  • Centre for Analysis and Synthesis
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2019-03-05

Language

English

Publication/Series

Metabolites

Volume

9

Issue

3

Document type

Journal article

Publisher

Multidisciplinary Digital Publishing Institute

Topic

  • Cell and Molecular Biology

Keywords

  • Cultured cells
  • Glutathione
  • Mass spectrometry
  • Oxidative stress
  • Yeast

Status

Published

ISBN/ISSN/Other

  • ISSN: 2218-1989