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Karl-Fredrik Eriksson

Associate professor

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Sorbitol and myo-inositol levels and morphology of sural nerve in relation to peripheral nerve function and clinical neuropathy in men with diabetic, impaired, and normal glucose tolerance

Author

  • Göran Sundkvist
  • L. B. Dahlin
  • H. Nilsson
  • K. F. Eriksson
  • F. Lindgärde
  • I. Rosén
  • S. A. Lattimer
  • A. A.F. Sima
  • K. Sullivan
  • D. A. Greene

Summary, in English

Aims: Sorbitol and myo-inositol levels and morphology of sural nerve were compared with nerve function and clinical neuropathy in men with diabetic, impaired (IGT), and normal glucose tolerance. Methods: After neurography of sural nerve and determinations of sensory thresholds for vibration, warm and cold on the foot, whole nerve sural nerve biopsy was performed in 10 men with Type 1 diabetes mellitus, 10 with IGT, and 10 with normal glucose tolerance. Polyol levels were assessed by gas-liquid chromatography/mass spectrometry. Results: Sural nerve amplitudes were significantly lower and sorbitol levels significantly higher in diabetic patients (median (interquartile range)) (3.7 (3.5) μV and 643 (412) pmol/mg protein, respectively) both compared with IGT (11.3 (10.6) μV; P = 0.04 and 286 (83) pmol/mg protein; P = 0.0032, respectively) and normally glucose tolerant (10.0 (11.6); P = 0.0142 and 296 (250) pmol/mg protein; P = 0.0191, respectively) subjects. There were no differences in nerve morphology between the three groups. Nerve myo-inositol levels correlated, however, positively with cluster density (r(s) = 0.56; P = 0.0054). In diabetic and IGT subjects, sural nerve amplitudes (2.6 (3.8) vs. 12.1 (10.6) μV; P = 0.0246) and myelinated nerve fibre density (MNFD; 4076 (1091) vs. 5219 (668) nerve fibres/mm2; P = 0.0021) were significantly lower in nine subjects with clinical neuropathy than in 10 without. Conclusions: Nerve degeneration (i.e. MNFD) correlated with clinical neuropathy but not with glucose tolerance status whereas nerve myo-inositol levels positively correlated with signs of nerve regeneration (i.e. increased cluster density).

Department/s

  • Department of Clinical Sciences, Malmö
  • WCMM-Wallenberg Centre for Molecular Medicine
  • Department of Translational Medicine
  • Hand Surgery, Malmö
  • Thoracic Surgery
  • Genomics, Diabetes and Endocrinology
  • Vascular Diseases - Clinical Research
  • Cardiovascular Research - Epidemiology
  • Clinical Neurophysiology

Publishing year

2000-04

Language

English

Pages

259-268

Publication/Series

Diabetic Medicine

Volume

17

Issue

4

Document type

Journal article

Publisher

Wiley-Blackwell

Topic

  • Endocrinology and Diabetes

Keywords

  • Human diabetes
  • IGT
  • Peripheral nerve function
  • Sorbitol
  • Sural nerve

Status

Published

Research group

  • Hand Surgery, Malmö
  • Genomics, Diabetes and Endocrinology
  • Vascular Diseases - Clinical Research
  • Cardiovascular Research - Epidemiology

ISBN/ISSN/Other

  • ISSN: 0742-3071