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ludc web

Joao Duarte

Research team manager

ludc web

Compartmentalised energy metabolism supporting glutamatergic neurotransmission in response to increased activity in the rat cerebral cortex : A 13C MRS study in vivo at 14.1 T

Author

  • Sarah Sonnay
  • João Mn Duarte
  • Nathalie Just
  • Rolf Gruetter

Summary, in English

Many tissues exhibit metabolic compartmentation. In the brain, while there is no doubt on the importance of functional compartmentation between neurons and glial cells, there is still debate on the specific regulation of pathways of energy metabolism at different activity levels. Using (13)C magnetic resonance spectroscopy (MRS) in vivo, we determined fluxes of energy metabolism in the rat cortex under α-chloralose anaesthesia at rest and during electrical stimulation of the paws. Compared to resting metabolism, the stimulated rat cortex exhibited increased glutamate-glutamine cycle (+67 nmol/g/min, +95%, P < 0.001) and tricarboxylic (TCA) cycle rate in both neurons (+62 nmol/g/min, +12%, P < 0.001) and astrocytes (+68 nmol/g/min, +22%, P = 0.072). A minor, non-significant modification of the flux through pyruvate carboxylase was observed during stimulation (+5 nmol/g/min, +8%). Altogether, this increase in metabolism amounted to a 15% (67 nmol/g/min, P < 0.001) increase in CMRglc(ox), i.e. the oxidative fraction of the cerebral metabolic rate of glucose. In conclusion, stimulation of the glutamate-glutamine cycle under α-chloralose anaesthesia is associated to similar enhancement of neuronal and glial oxidative metabolism.

Publishing year

2016-05

Language

English

Pages

40-928

Publication/Series

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Volume

36

Issue

5

Document type

Journal article

Publisher

Nature Publishing Group

Keywords

  • Animals
  • Astrocytes
  • Carbon Isotopes
  • Cell Compartmentation
  • Cerebral Cortex
  • Electric Stimulation
  • Energy Metabolism
  • Glutamic Acid
  • Magnetic Resonance Imaging
  • Neuroglia
  • Neurons
  • Rats
  • Synaptic Transmission
  • Journal Article

Status

Published

ISBN/ISSN/Other

  • ISSN: 1559-7016