The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

ludc web

Joao Duarte

Principal investigator

ludc web

Characterization of cerebral glucose dynamics in vivo with a four-state conformational model of transport at the blood-brain barrier

Author

  • João M N Duarte
  • Rolf Gruetter

Summary, in English

Determination of brain glucose transport kinetics in vivo at steady-state typically does not allow distinguishing apparent maximum transport rate (T(max)) from cerebral consumption rate. Using a four-state conformational model of glucose transport, we show that simultaneous dynamic measurement of brain and plasma glucose concentrations provide enough information for independent and reliable determination of the two rates. In addition, although dynamic glucose homeostasis can be described with a reversible Michaelis-Menten model, which is implicit to the large iso-inhibition constant (K(ii)) relative to physiological brain glucose content, we found that the apparent affinity constant (K(t)) was better determined with the four-state conformational model of glucose transport than with any of the other models tested. Furthermore, we confirmed the utility of the present method to determine glucose transport and consumption by analysing the modulation of both glucose transport and consumption by anaesthesia conditions that modify cerebral activity. In particular, deep thiopental anaesthesia caused a significant reduction of both T(max) and cerebral metabolic rate for glucose consumption. In conclusion, dynamic measurement of brain glucose in vivo in function of plasma glucose allows robust determination of both glucose uptake and consumption kinetics.

Publishing year

2012-05

Language

English

Pages

396-406

Publication/Series

Journal of Neurochemistry

Volume

121

Issue

3

Document type

Journal article

Publisher

Wiley-Blackwell

Keywords

  • Algorithms
  • Anesthesia
  • Anesthetics
  • Animals
  • Biological Transport, Active
  • Blood Glucose
  • Blood-Brain Barrier
  • Brain Chemistry
  • Glucose
  • Homeostasis
  • Kinetics
  • Magnetic Resonance Spectroscopy
  • Male
  • Models, Biological
  • Rats
  • Rats, Sprague-Dawley

Status

Published

ISBN/ISSN/Other

  • ISSN: 1471-4159