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ludc web

Joao Duarte

Principal investigator

ludc web

The C57BL/6J mouse exhibits sporadic congenital portosystemic shunts

Author

  • Cristina Cudalbu
  • Valérie A McLin
  • Hongxia Lei
  • Joao M N Duarte
  • Anne-Laure Rougemont
  • Graziano Oldani
  • Sylvain Terraz
  • Christian Toso
  • Rolf Gruetter

Summary, in English

C57BL/6 mice are the most widely used strain of laboratory mice. Using in vivo proton Magnetic Resonance Spectroscopy ((1)H MRS), we have repeatedly observed an abnormal neurochemical profile in the brains of both wild-type and genetically modified mice derived from the C57BL/6J strain, consisting of a several fold increase in cerebral glutamine and two fold decrease in myo-inositol. This strikingly abnormal neurochemical "phenotype" resembles that observed in chronic liver disease or portosystemic shunting and appeared to be independent of transgene, origin or chow and was not associated with liver failure. As many as 25% of animals displayed the abnormal neurochemical profile, questioning the reliability of this model for neurobiology. We conducted an independent study to determine if this neurochemical profile was associated with portosystemic shunting. Our results showed that 100% of the mice with high brain glutamine displayed portosystemic shunting by concomitant portal angiography while all mice with normal brain glutamine did not. Since portosystemic shunting is known to cause alterations in gene expression in many organs including the brain, we conclude that portosystemic shunting may be the most significant problem associated with C57BL/6J inbreeding both for its effect on the central nervous system and for its systemic repercussions.

Publishing year

2013

Language

English

Pages

69782-69782

Publication/Series

PLoS ONE

Volume

8

Issue

7

Document type

Journal article

Publisher

Public Library of Science (PLoS)

Keywords

  • Angiography
  • Animals
  • Brain
  • Glutamine
  • Immunohistochemistry
  • Magnetic Resonance Spectroscopy
  • Mice
  • Mice, Inbred C57BL
  • Portasystemic Shunt, Surgical
  • Journal Article
  • Research Support, Non-U.S. Gov't

Status

Published

ISBN/ISSN/Other

  • ISSN: 1932-6203