The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

ludc web

Joao Duarte

Principal investigator

ludc web

High-fat diet consumption alters energy metabolism in the mouse hypothalamus

Author

  • Blanca Lizarbe
  • Antoine Cherix
  • João M.N. Duarte
  • Jean René Cardinaux
  • Rolf Gruetter

Summary, in English

Background/Objectives: High-fat diet consumption is known to trigger an inflammatory response in the hypothalamus, which has been characterized by an initial expression of pro-inflammatory genes followed by hypothalamic astrocytosis, microgliosis, and the appearance of neuronal injury markers. The specific effects of high-fat diet on hypothalamic energy metabolism and neurotransmission are however not yet known and have not been investigated before. Subjects/Methods: We used 1H and 13C magnetic resonance spectroscopy (MRS) and immunofluorescence techniques to evaluate in vivo the consequences of high-saturated fat diet administration to mice, and explored the effects on hypothalamic metabolism in three mouse cohorts at different time points for up to 4 months. Results: We found that high-fat diet increases significantly the hypothalamic levels of glucose (P < 0.001), osmolytes (P < 0.001), and neurotransmitters (P < 0.05) from 2 months of diet, and alters the rates of metabolic (P < 0.05) and neurotransmission fluxes (P < 0.001), and the contribution of non-glycolytic substrates to hypothalamic metabolism (P < 0.05) after 10 weeks of high-fat feeding. Conclusions/interpretation: We report changes that reveal a high-fat diet-induced alteration of hypothalamic metabolism and neurotransmission that is quantifiable by 1H and 13C MRS in vivo, and present the first evidence of the extension of the inflammation pathology to a localized metabolic imbalance.

Department/s

  • WCMM-Wallenberg Centre for Molecular Medicine
  • Diabetes and Brain Function
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2019

Language

English

Pages

1295-1304

Publication/Series

International Journal of Obesity

Volume

43

Issue

6

Document type

Journal article

Publisher

Nature Publishing Group

Topic

  • Physiology
  • Nutrition and Dietetics

Status

Published

Research group

  • Diabetes and Brain Function

ISBN/ISSN/Other

  • ISSN: 0307-0565