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ludc web

Joao Duarte

Research team manager

ludc web

MRS glucose mapping and PET joining forces : re-evaluation of the lumped constant in the rat brain under isoflurane anaesthesia

Author

  • Malte F Alf
  • João M N Duarte
  • Hongxia Lei
  • Stefanie D Krämer
  • Vladimir Mlynarik
  • Roger Schibli
  • Rolf Gruetter

Summary, in English

Although numerous positron emission tomography (PET) studies with (18) F-fluoro-deoxyglucose (FDG) have reported quantitative results on cerebral glucose kinetics and consumption, there is a large variation between the absolute values found in the literature. One of the underlying causes is the inconsistent use of the lumped constants (LCs), the derivation of which is often based on multiple assumptions that render absolute numbers imprecise and errors hard to quantify. We combined a kinetic FDG-PET study with magnetic resonance spectroscopic imaging (MRSI) of glucose dynamics in Sprague-Dawley rats to obtain a more comprehensive view of brain glucose kinetics and determine a reliable value for the LC under isoflurane anaesthesia. Maps of Tmax /CMRglc derived from MRSI data and Tmax determined from PET kinetic modelling allowed to obtain an LC-independent CMRglc . The LC was estimated to range from 0.33 ± 0.07 in retrosplenial cortex to 0.44 ± 0.05 in hippocampus, yielding CMRglc between 62 ± 14 and 54 ± 11 μmol/min/100 g, respectively. These newly determined LCs for four distinct areas in the rat brain under isoflurane anaesthesia provide means of comparing the growing amount of FDG-PET data available from translational studies.

Publishing year

2014-05

Language

English

Pages

82-672

Publication/Series

Journal of Neurochemistry

Volume

129

Issue

4

Document type

Journal article

Publisher

Wiley-Blackwell

Keywords

  • Algorithms
  • Anesthetics, Inhalation
  • Animals
  • Biological Transport
  • Brain
  • Brain Chemistry
  • Cerebral Cortex
  • Fluorine Radioisotopes
  • Fluorodeoxyglucose F18
  • Glucose
  • Hippocampus
  • Isoflurane
  • Magnetic Resonance Spectroscopy
  • Models, Biological
  • Multimodal Imaging
  • Positron-Emission Tomography
  • Radiopharmaceuticals
  • Rats
  • Rats, Sprague-Dawley
  • Thalamus
  • Comparative Study
  • Journal Article
  • Research Support, Non-U.S. Gov't

Status

Published

ISBN/ISSN/Other

  • ISSN: 1471-4159