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Jens Lagerstedt

Associate professor

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Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89

Author

  • Palanivelu Sengottaiyan
  • Jitka Petrlova
  • Jens O Lagerstedt
  • Lorena Ruiz-Pavon
  • Madhu S. Budamagunta
  • John C Voss
  • Bengt L Persson

Summary, in English

In Saccharomyces cerevisiae, Pho89 mediates a cation-dependent transport of Pi across the plasma membrane. This integral membrane protein belongs to the Inorganic Phosphate Transporter (PiT) family, a group that includes the mammalian Na(+)/Pi cotransporters Pit1 and Pit2. Here we report that the Pichia pastoris expressed recombinant Pho89 was purified in the presence of Foscholine-12 and functionally reconstituted into proteoliposomes with a similar substrate specificity as observed in an intact cell system. The alpha-helical content of the Pho89 protein was estimated to 44%. EPR analysis showed that purified Pho89 protein undergoes conformational change upon addition of substrate.

Department/s

  • Department of Experimental Medical Science

Publishing year

2013-07-05

Language

English

Pages

551-556

Publication/Series

Biochemical and Biophysical Research Communications

Volume

436

Issue

3

Document type

Journal article

Publisher

Elsevier

Topic

  • Biological Sciences

Keywords

  • Pho89
  • Pichia pastoris
  • Oligomer
  • Reconstitution
  • Phosphate transport
  • Circular dichroism

Status

Published

ISBN/ISSN/Other

  • ISSN: 1090-2104