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Jens Lagerstedt

Associate professor

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Intracellular localization of an active green fluorescent protein-tagged Pho84 phosphate permease in Saccharomyces cerevisiae

Author

  • Jens Petersson
  • Johanna Pattison
  • Arthur L Kruckeberg
  • Jan A Berden
  • B L Persson

Summary, in English

Green fluorescent protein (GFP) from Aequorea victoria was used as an in vivo reporter protein when fused to the carboxy-terminus of the Pho84 phosphate permease of Saccharomyces cerevisiae. Both components of the fusion protein displayed their native functions and revealed a cellular localization and degradation of the Pho84-GFP chimera consistent with the behavior of the wild-type Pho84 protein. The GFP-tagged chimera allowed for a detection of conditions under which the Pho84 transporter is localized to its functional environment, i.e. the plasma membrane, and conditions linked to relocation of the protein to the vacuole for degradation. By use of the methodology described, GFP should be useful in studies of localization and degradation also of other membrane proteins in vivo.

Publishing year

1999

Language

English

Pages

37-42

Publication/Series

FEBS Letters

Volume

462

Issue

1-2

Document type

Journal article

Publisher

Wiley-Blackwell

Keywords

  • Carrier Proteins/biosynthesis
  • Cell Membrane/metabolism
  • Fungal Proteins/biosynthesis
  • Green Fluorescent Proteins
  • Luminescent Proteins/genetics
  • Phosphates/physiology
  • Proton-Phosphate Symporters
  • Recombinant Fusion Proteins/biosynthesis
  • Saccharomyces cerevisiae/enzymology
  • Saccharomyces cerevisiae Proteins

Status

Published

ISBN/ISSN/Other

  • ISSN: 0014-5793