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Jens Lagerstedt

Associate professor

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Imaging apolipoprotein AI in vivo

Author

  • Renuka Sriram
  • Jens Lagerstedt
  • Jitka Petrlova
  • Haris Samardzic
  • Ulrike Kreutzer
  • Hongtao Xie
  • George A. Kaysen
  • Jean F. Desreux
  • David Thonon
  • Vincent Jacques
  • Martha Van Loan
  • John C. Rutledge
  • Michael N. Oda
  • John C. Voss
  • Thomas Jue

Summary, in English

Coronary disease risk increases inversely with high-density lipoprotein (HDL) level. The measurement of the biodistribution and clearance of HDL in vivo, however, has posed a technical challenge. This study presents an approach to the development of a lipoprotein MRI agent by linking gadolinium methanethiosulfonate (Gd[MTS-ADO3A]) to a selective cysteine mutation in position 55 of apo AI, the major protein of HDL. The contrast agent targets both liver and kidney, the sites of HDL catabolism, whereas the standard MRI contrast agent, gadolinium-diethylenetriaminepentaacetic acid-bismethylamide (GdDTPA-BMA, gadodiamide), enhances only the kidney image. Using a modified apolipoprotein AI to create an HDL contrast agent provides a new approach to investigate HDL biodistribution, metabolism and regulation in vivo. Copyright (C) 2011 John Wiley & Sons, Ltd.

Department/s

  • Cellular Biomechanics
  • EXODIAB: Excellence in Diabetes Research in Sweden

Publishing year

2011

Language

English

Pages

916-924

Publication/Series

NMR in Biomedicine

Volume

24

Issue

7

Document type

Journal article

Publisher

John Wiley and Sons

Topic

  • Radiology, Nuclear Medicine and Medical Imaging

Keywords

  • NMR
  • apolipoprotein
  • HDL
  • contrast agent
  • MRI
  • gadolinium
  • cardiovascular risk

Status

Published

Research group

  • Cellular Biomechanics

ISBN/ISSN/Other

  • ISSN: 0952-3480