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Jens Lagerstedt

Associate professor

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Phosphate permeases of Saccharomyces cerevisiae

Author

  • Persson B. L.
  • A. Berhe
  • U. Fristedt
  • P. Martinez
  • J. Pattison
  • Jens Petersson
  • R. Weinander

Summary, in English

The PHO84 and PHO89 genes of Saccharomyces cerevisiae encode two high- affinity phosphate cotransporters of the plasma membrane. Hydropathy analysis suggests a secondary structure arrangements of the proteins in 12 transmembrane domains. The derepressible Pho84 and Pho89 transporters appear to have characteristic similarities with the phosphate transporters of Neurospora crassa. The Pho84 protein catalyzes a proton-coupled phosphate transport at acidic pH, while the Pho89 protein catalyzes a sodium-dependent phosphate uptake at alkaline pH. The Pho84 transporter can be stably overproduced in the cytoplasmic membrane of Escherichia coli, purified and reconstituted in a functional state into proteoliposomes.

Publishing year

1998-06-10

Language

English

Pages

23-30

Publication/Series

Biochimica et Biophysica Acta - Bioenergetics

Volume

1365

Issue

1-2

Document type

Journal article

Publisher

Elsevier

Topic

  • Biochemistry and Molecular Biology

Keywords

  • PHO regulation
  • Phosphate transporter
  • Plasma membrane transport
  • Saccharomyces cerevisiae

Status

Published

ISBN/ISSN/Other

  • ISSN: 0005-2728