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Jens Lagerstedt

Associate professor

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Molecular mechanisms controlling phosphate-induced downregulation of the yeast Pho84 phosphate transporter

Author

  • Fredrik Lundh
  • Jean-Marie Mouillon
  • Dieter Samyn
  • Kent Stadler
  • Yulia Popova
  • Jens O Lagerstedt
  • Johan M Thevelein
  • Bengt L Persson

Summary, in English

In Saccharomyces cerevisiae, phosphate uptake is mainly dependent on the proton-coupled Pho84 permease under phosphate-limited growth conditions. Phosphate addition causes Pho84-mediated activation of the protein kinase A (PKA) pathway as well as rapid internalization and vacuolar breakdown of Pho84. We show that Pho84 undergoes phosphate-induced phosphorylation and subsequent ubiquitination on amino acids located in the large middle intracellular loop prior to endocytosis. The attachment of ubiquitin is dependent on the ubiquitin conjugating enzymes Ubc2 and Ubc4. In addition, we show that the Pho84 endocytotic process is delayed in strains with reduced PKA activity. Our results suggest that Pho84-mediated activation of the PKA pathway is responsible for its own downregulation by phosphorylation, ubiquination, internalization, and vacuolar breakdown.

Publishing year

2009-06-02

Language

English

Pages

505-4497

Publication/Series

Biochemistry

Volume

48

Issue

21

Document type

Journal article

Publisher

The American Chemical Society (ACS)

Keywords

  • Cyclic AMP-Dependent Protein Kinases
  • Down-Regulation
  • Feedback, Physiological
  • Intracellular Space
  • Phosphates
  • Phosphorylation
  • Protein Transport
  • Proton-Phosphate Symporters
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Signal Transduction
  • Ubiquitin
  • Up-Regulation
  • Journal Article
  • Research Support, Non-U.S. Gov't

Status

Published

ISBN/ISSN/Other

  • ISSN: 0006-2960