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Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells

  • Polyxeni T. Mantani
  • Jenifer Vallejo
  • Irena Ljungcrantz
  • Jan Nilsson
  • Harry Björkbacka
  • Gunilla Nordin Fredrikson
Publishing year: 2018-01-01
Language: English
Pages: 685-692
Publication/Series: Human Immunology
Volume: 79
Issue: 9
Document type: Journal article
Publisher: Elsevier

Abstract english

Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly expressed on T cells. Incubation of hPBMCs with IL-25 reduced the frequency of Th17 cells. Furthermore, IL-25 inhibited the release of the Th17-inducing cytokine IL-6 from dendritic cells isolated from hPBMCs indicating that the IL-25 mediated Th17 suppression may be indirect. Moreover, IL-25 reduced the secretion of the proinflammatory cytokine IFNγ from hPBMCs. OxLDL decreased IFNγ release from hPBMCs regardless of the presence or absence of IL-25. Conclusions: IL-25 reduces Th1 and Th17 immune responses in hPBMCs raising the interesting possibility that IL-25 could have a protective role in human atherosclerosis.


  • Immunology in the medical area
  • IL-25
  • Oxidized LDL
  • Th17


  • Cardiovascular Research - Cellular Metabolism and Inflammation
  • ISSN: 0198-8859
E-mail: jan [dot] nilsson [at] med [dot] lu [dot] se

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