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Jan Nilsson

Professor

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Interleukin-25 reduces Th17 cells and inflammatory responses in human peripheral blood mononuclear cells

Author

  • Polyxeni T. Mantani
  • Jenifer Vallejo
  • Irena Ljungcrantz
  • Jan Nilsson
  • Harry Björkbacka
  • Gunilla Nordin Fredrikson

Summary, in English

Background: The absence of interleukin-25 (IL-25) favors the induction of Th1 and Th17 immune responses in mice. Th1 immune responses have been associated with the pathology of atherosclerosis, a lipid and inflammation driven disease of the arterial wall. Purpose of research: To evaluate the effect of IL-25 on human peripheral blood mononuclear cells (hPBMCs) in the presence and absence of oxidized low density lipoprotein (oxLDL), a key player in atherosclerosis development. Principal results: Human PBMCs were incubated with recombinant human IL-25 (rhIL-25) in the presence and absence of oxLDL and analyzed with flow cytometry while cytokine secretion was measured in cell culture supernatants. The IL-25 receptor, IL-17RB, was mostly expressed on T cells. Incubation of hPBMCs with IL-25 reduced the frequency of Th17 cells. Furthermore, IL-25 inhibited the release of the Th17-inducing cytokine IL-6 from dendritic cells isolated from hPBMCs indicating that the IL-25 mediated Th17 suppression may be indirect. Moreover, IL-25 reduced the secretion of the proinflammatory cytokine IFNγ from hPBMCs. OxLDL decreased IFNγ release from hPBMCs regardless of the presence or absence of IL-25. Conclusions: IL-25 reduces Th1 and Th17 immune responses in hPBMCs raising the interesting possibility that IL-25 could have a protective role in human atherosclerosis.

Department/s

  • Department of Clinical Sciences, Malmö
  • EpiHealth: Epidemiology for Health
  • Cardiovascular Research - Cellular Metabolism and Inflammation
  • EXODIAB: Excellence of Diabetes Research in Sweden

Publishing year

2018-01-01

Language

English

Pages

685-692

Publication/Series

Human Immunology

Volume

79

Issue

9

Document type

Journal article

Publisher

Elsevier

Topic

  • Immunology in the medical area

Keywords

  • IL-25
  • Oxidized LDL
  • Th17

Status

Published

Research group

  • Cardiovascular Research - Cellular Metabolism and Inflammation

ISBN/ISSN/Other

  • ISSN: 0198-8859