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Inhibitory effect on arterial injury-induced neointimal formation by adoptive B-cell transfer in Rag-1 knockout mice.

Author:
  • Paul Dimayuga
  • Bojan Cercek
  • Sumito Oguchi
  • Gunilla Nordin Fredrikson
  • Juliana Yano
  • Prediman K Shah
  • Stefan Jovinge
  • Jan Nilsson
Publishing year: 2002
Language: English
Pages: 9-644
Publication/Series: Arteriosclerosis, Thrombosis and Vascular Biology
Volume: 22
Issue: 4
Document type: Journal article
Publisher: Lippincott Williams Wilkins Hagerstown, MD

Abstract english

We investigated the effect of B-cell reconstitution in immune-deficient Rag-1 knockout (KO) mice subjected to arterial injury. After 21 days, injury induced a 4- to 5-fold increase in neointimal formation in Rag-1 KO mice fed normal chow compared with wild-type (WT) mice (0.020+/-0.0160 [n=8] versus 0.0049+/-0.0022 [n=8] mm(2), respectively; P<0.05) and in western-type diet-fed Rag-1 KO mice compared with WT mice (0.0312+/-0.0174 [n=7] versus 0.0050+/-0.0028 [n=6] mm(2), respectively; P<0.05). To investigate the role of B cells in response to injury, Rag-1 KO mice were reconstituted with B cells derived from the spleens of WT mice, with donors and recipients on the same diet. Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed normal chow) reduced neointimal formation compared with the effect in unreconstituted Rag-1 KO mice (0.0076+/-0.0039 [n=9] versus 0.020+/-0.0160 [n=8] mm(2), respectively; P<0.05). Reconstitution of Rag-1 KO mice with B cells from WT mice (both fed a western diet) reduced neointimal formation compared the effect in Rag-1 KO mice (0.0087+/-0.0037 [n=8] versus 0.0312+/-0.0174 [n=7] mm(2), respectively; P<0.05). Injured carotid arteries from reconstituted Rag-1 KO mice had detectable IgM and IgG, indicating viable transfer of B cells. The results suggest that B cells modulate the response to arterial injury.

Keywords

  • Cardiac and Cardiovascular Systems
  • Knockout
  • Mice
  • Male
  • Immunoglobulin M : blood
  • Immunoglobulin G : blood
  • Cellular
  • Immunity
  • Homeodomain Proteins : drug effects
  • Diet
  • Cholesterol : blood
  • Carotid Arteries : pathology
  • Carotid Arteries : immunology
  • B-Lymphocytes : transplantation
  • B-Lymphocytes : immunology
  • Adoptive Transfer
  • Animal
  • Models
  • Support
  • Non-U.S. Gov't
  • Tunica Intima : growth & development
  • Tunica Intima : immunology
  • Tunica Intima : injuries

Other

Published
  • Cardiovascular Research - Immunity and Atherosclerosis
  • ISSN: 1524-4636
E-mail: jan [dot] nilsson [at] med [dot] lu [dot] se

Lund University Diabetes Centre, CRC, SUS Malmö, Jan Waldenströms gata 35, House 91:12. SE-214 28 Malmö. Telephone: +46 40 39 10 00