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Nanomolar concentrations of lysophosphatidylcholine recruit monocytes and induce pro-inflammatory cytokine production in macrophages.

Author:
  • Katarina Berg
  • Linda Andersson
  • Jan Nilsson
  • Harry Björkbacka
Publishing year: 2008
Language: English
Pages: 348-352
Publication/Series: Biochemical and Biophysical Research Communications
Volume: 370
Document type: Journal article
Publisher: Elsevier

Abstract english

Lysophosphatidylcholine (LPC) has been attributed a pro-inflammatory role in atherosclerosis. Cell culture studies have identified stimulation of cytokine expression and chemotaxis by micromolar (muM) concentrations of LPC. In the present study we have investigated if LPC, in similarity with many other lipid mediators, has pro-inflammatory effects also at nanomolar (nM) concentrations. Cultured mouse bone marrow derived and RAW264.7 macrophages exposed to LPC demonstrated two peaks of increased MIP-2 release and mRNA expression; one at 0.1-10nM and another at muM concentrations. Both concentration ranges of LPC were also found to stimulate THP-1 monocyte chemotaxis. However, stimulation of the cells with muM concentrations of LPC may cause cell injury as increased release of lactate dehydrogenase was observed. Our findings demonstrate two peaks of LPC-induced pro-inflammatory activity, one in the nM and one in the muM range, and indicate that the latter may involve a stress response to lipid cytotoxicity.

Keywords

  • Biological Sciences

Other

Published
  • Cardiovascular Research - Immunity and Athersosclerosis
  • ISSN: 1090-2104
E-mail: jan [dot] nilsson [at] med [dot] lu [dot] se

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